Gene Expression Changes in the Course of Neural Progenitor Cell Differentiation

Overview

Journal J Neurosci

Specialty Neurology

Date 2004 Jul 2

PMID 15229246

Citations 38

Authors

Ulf Gurok

Christine Steinhoff

Bettina Lipkowitz

H-Hilger Ropers

Constance Scharff

Ulrike A Nuber

Affiliations

Soon will be listed here.

Abstract

The molecular changes underlying neural progenitor differentiation are essentially unknown. We applied cDNA microarrays with 13,627 clones to measure dynamic gene expression changes during the in vitro differentiation of neural progenitor cells that were isolated from the subventricular zone of postnatal day 7 mice and grown in vitro as neurospheres. In two experimental series in which we withdrew epidermal growth factor and added the neurotrophins Neurotrophin-4 or BDNF, four time points were investigated: undifferentiated cells grown as neurospheres, and cells 24, 48, and 96 hr after differentiation. Expression changes of selected genes were confirmed by semiquantitative RT-PCR. Ten different groups of gene expression dynamics obtained by cluster analysis are described. To correlate selected gene expression changes to the localization of respective proteins, we performed immunostainings of cultured neurospheres and of brain sections from adult mice. Our results provide new insights into the genetic program of neural progenitor differentiation and give strong hints to as yet unknown cellular communications within the adult subventricular zone stem cell niche.

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