Loss of Heterozygosity on Chromosome 10q22-10q23 and 22q11.2-22q12.1 and P53 Gene in Primary Hepatocellular Carcinoma

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2004 Jun 29

PMID 15222050

Citations 10

Authors

Guang-Neng Zhu

Li Zuo

Qing Zhou

Su-Mei Zhang

Hua-Qing Zhu

Shu-Yu Gui

Yuan Wang

Affiliations

Soon will be listed here.

Abstract

Aim: To analyze loss of heterozygosity (LOH) and homozygous deletion on p53 gene (exon2-3, 4 and 11), chromosome 10q22-10q23 and 22q11.2 -22q12.1 in human hepatocellular carcinoma (HCC).

Methods: PCR and PCR-based microsatellite polymorphism analysis techniques were used.

Results: LOH was observed at D10S579 (10q22-10q23) in 4 of 20 tumors (20%), at D22S421 (22q11.2-22q12.1) in 3 of 20(15%), at TP53.A (p53 gene exon 2-3) in 4 of 20(20%), at TP53.B (p53 gene exon 4) in 6 of 20(30%), and at TP53.G (p53 gene exon 11) in 0 of 20(0%). Homozygous deletion was detected at 10q22-10q23(8/20; 40%), 22q11.2-22q12.1(8/20; 40%), p53 gene exon 2-3(0/20;0%), p53 gene exon 4(6/20; 30%), and p53 gene exon 11(2/20; 10%).

Conclusion: There might be unidentified tumor suppressor genes on chromosome 10q22-10q23 and 22q11.2-22q12.1 that contribute to the pathogenesis and development of HCC.

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