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Echinococcus Multilocularis Proliferation in Mice and Respective Parasite 14-3-3 Gene Expression is Mainly Controlled by an Alphabeta CD4 T-cell-mediated Immune Response

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Journal Immunology
Date 2004 Jun 16
PMID 15196217
Citations 12
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Abstract

The role of specific B lymphocytes and T-cell populations in the control of experimental Echinococus multilocularis infection was studied in micro MT, nude, T-cell receptor (TCR)-beta(-/-), major histocompatibility complex (MHC)-I(-/-) and MHC-II(-/-) mice. At 2 months postinfection, the parasite mass was more than 10 times higher in nude, TCR-beta(-/-) and MHC-II(-/-) mice than in infected C57BL/6 wild-type (WT) mice, and these T-cell-deficient mice started to die of the high parasite load at this time-point. In contrast, MHC-I(-/-) and micro MT mice exhibited parasite growth rates similar to those found in WT controls. These findings clearly point to the major role that CD4(+) alphabeta(+) T cells play in limiting the E. multilocularis proliferation, while CD8(+) T and B cells appeared to play a minor role in the control of parasite growth. In the absence of T cells, especially CD4(+) or alphabeta(+) T cells, the cellular immune response to infection was impaired, as documented by the lack of hepatic granuloma formation around the parasite and by a decreased splenocyte responsiveness to concanavalin A (Con A) and parasite antigen stimulation. Surprisingly, in T-cell-deficient mice, the ex vivo expression of interferon-gamma (IFN-gamma) and other inflammatory cytokines (except for interleukin-6) were increased in association with a high parasite load. Thus, the relative protection mediated by CD4(+) alphabeta(+) T cells against E. multilocularis infection seemed not be IFN-gamma dependent, but rather to rely on the effector's function of CD4(+) alphabeta(+) T cells. The local restriction of parasite germinal cell proliferation was reflected by a regulatory effect on the expression of 14-3-3 protein within the parasite tissue in T-cell-deficient mice. These results provide a strong indication that the CD4(+) alphabeta(+) T-cell-mediated immune response contributes to the control of the parasite growth and to the regulation of production of the parasite 14-3-3 protein in metacestode tissues.

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References
1.
Bresson-Hadni S, Liance M, Meyer J, Houin R, Bresson J, Vuitton D . Cellular immunity in experimental Echinococcus multilocularis infection. II. Sequential and comparative phenotypic study of the periparasitic mononuclear cells in resistant and sensitive mice. Clin Exp Immunol. 1990; 82(2):378-83. PMC: 1535102. DOI: 10.1111/j.1365-2249.1990.tb05457.x. View

2.
Vuitton D, Bresson-Hadni S, Laroche L, Kaiserlian D, Bresson J, Gillet M . Cellular immune response in Echinococcus multilocularis infection in humans. II. Natural killer cell activity and cell subpopulations in the blood and in the periparasitic granuloma of patients with alveolar echinococcosis. Clin Exp Immunol. 1989; 78(1):67-74. PMC: 1534601. View

3.
Playford M, Ooi H, Oku Y, Kamiya M . Secondary Echinococcus multilocularis infection in severe combined immunodeficient (scid) mice: biphasic growth of the larval cyst mass. Int J Parasitol. 1992; 22(7):975-82. DOI: 10.1016/0020-7519(92)90056-q. View

4.
Playford M, Kamiya M . Immune response to Echinococcus multilocularis infection in the mouse model: a review. Jpn J Vet Res. 1992; 40(2-3):113-30. View

5.
Gottstein B, Wunderlin E, Tanner I . Echinococcus multilocularis: parasite-specific humoral and cellular immune response subsets in mouse strains susceptible (AKR, C57B1/6J) or 'resistant' (C57B1/10) to secondary alveolar echinococcosis. Clin Exp Immunol. 1994; 96(2):245-52. PMC: 1534874. DOI: 10.1111/j.1365-2249.1994.tb06549.x. View