Polymorphisms of Interleukin-1B and Interleukin-1 Receptor Antagonist Genes in Patients with Chronic Hepatitis B

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2004 Jun 10

PMID 15188516

Citations 9

Authors

Ping-An Zhang

Yan Li

Pu Xu

Jian-Min Wu

Affiliations

Soon will be listed here.

Abstract

Aim: To investigate the relationships between polymorphisms of interleukin-1B (IL-1B) promoter region -511C/T and interleukin-1 receptor antagonist gene (IL-1RN) and susceptibility to chronic hepatitis B in Chinese population.

Methods: Genomic DNA was extracted from the peripheral blood of 190 patients with chronic hepatitis B and 249 normal controls and then subjected to polymerase chain reaction (PCR) amplification. The PCR products were digested by restriction endonuclease AvaI. The products of digestion were subjected to 20 g/L gel electrophoresis and ethidium bromide staining.

Results: The frequencies of IL-1B (-511) genotypes CC, CT and TT in patients with chronic hepatitis B were 23.7%, 49.5% and 26.8%, while 26.1%, 47.4% and 26.5% respectively in controls. The results showed that there was no significant difference in the frequencies of alleles or genotypes in IL-1B between patients with chronic hepatitis B and controls. The distributions of IL-1B (-511) genotype CC were significantly different between the two subgroups (HBV-DNA </=1x10(3) copies/mL as subgroup I, HBV-DNA >1x10(3) copies/mL as subgroup II) of chronic hepatitis B (P=0.029). Only four of the five kinds of polymorphism (1/1, 1/2, 2/2 and 1/4) were found in this study. The frequencies of IL-1RN genotypes 1/1, 1/2, 2/2 and 1/4 were 88.9%, 9.0%, 0.5% and 1.6% in patients with chronic hepatitis B respectively, while were 81.1%, 16.9%, 0.4% and 1.6% respectively in controls. The frequencies of genotype1/2 and IL-1RN allele 2 in patients with chronic hepatitis B were lower than those in controls (P=0.016 and P=0.029, respectively).

Conclusion: There is an association between polymorphisms of the promoter region -511C/T of IL-1B and IL-1RN intron 2 and chronic hepatitis B virus infection. Subjects with IL-1RN allele 2 may be resistant to HBV infection, and IL-1B(-511)genotype CC is closely related with HBV-DNA replication, which gives some new clues to the study of pathogenesis of chronic hepatitis B.

Citing Articles

Host and Viral Factors Influencing Chronic Hepatitis B Infection Across Three Generations in a Family.

Naderi M, Hosseini S, Behnampour N, Besharat S, Shahramian I, Khoshnia M Curr Microbiol. 2024; 81(12):446.

PMID: 39499325 DOI: 10.1007/s00284-024-03963-8.

Dondeti M, El-Maadawy E, Talaat R World J Gastroenterol. 2016; 22(30):6800-16.

PMID: 27570418 PMC: 4974580. DOI: 10.3748/wjg.v22.i30.6800.

Th1/Th2 cytokines and their genotypes as predictors of hepatitis B virus related hepatocellular carcinoma.

Saxena R, Kaur J World J Hepatol. 2015; 7(11):1572-80.

PMID: 26085916 PMC: 4462695. DOI: 10.4254/wjh.v7.i11.1572.

Association of Interleukin-1β and Gene Polymorphisms with Liver Pathogenesis in Hepatitis B Virus Infection among Eastern Indian Population.

Biswas A, Panigrahi R, Pal M, De B, Chakrabarti S, Ghosh M J Clin Exp Hepatol. 2015; 3(4):281-7.

PMID: 25755515 PMC: 3940093. DOI: 10.1016/j.jceh.2013.11.006.

Relationship between cytokine gene polymorphisms and chronic hepatitis B virus infection.

Tuncbilek S World J Gastroenterol. 2014; 20(20):6226-35.

PMID: 24876743 PMC: 4033460. DOI: 10.3748/wjg.v20.i20.6226.

References

Cacciola I, Cerenzia G, Pollicino T, Squadrito G, Castellaneta S, Zanetti A . Genomic heterogeneity of hepatitis B virus (HBV) and outcome of perinatal HBV infection. J Hepatol. 2002; 36(3):426-32. DOI: 10.1016/s0168-8278(01)00295-1. View

Hutyrova B, Pantelidis P, Drabek J, Zurkova M, Kolek V, Lenhart K . Interleukin-1 gene cluster polymorphisms in sarcoidosis and idiopathic pulmonary fibrosis. Am J Respir Crit Care Med. 2002; 165(2):148-51. DOI: 10.1164/ajrccm.165.2.2106004. View

Koziel M . Cytokines in viral hepatitis. Semin Liver Dis. 1999; 19(2):157-69. DOI: 10.1055/s-2007-1007107. View

Giedraitis V, He B, Huang W, Hillert J . Cloning and mutation analysis of the human IL-18 promoter: a possible role of polymorphisms in expression regulation. J Neuroimmunol. 2000; 112(1-2):146-52. DOI: 10.1016/s0165-5728(00)00407-0. View

Wang F . Current status and prospects of studies on human genetic alleles associated with hepatitis B virus infection. World J Gastroenterol. 2003; 9(4):641-4. PMC: 4611419. DOI: 10.3748/wjg.v9.i4.641. View

Pociot F, Molvig J, Wogensen L, Worsaae H, Nerup J . A TaqI polymorphism in the human interleukin-1 beta (IL-1 beta) gene correlates with IL-1 beta secretion in vitro. Eur J Clin Invest. 1992; 22(6):396-402. DOI: 10.1111/j.1365-2362.1992.tb01480.x. View

Moos V, Rudwaleit M, Herzog V, Hohlig K, Sieper J, Muller B . Association of genotypes affecting the expression of interleukin-1beta or interleukin-1 receptor antagonist with osteoarthritis. Arthritis Rheum. 2000; 43(11):2417-22. DOI: 10.1002/1529-0131(200011)43:11<2417::AID-ANR7>3.0.CO;2-R. View

Ben-Ari Z, Mor E, Papo O, Kfir B, Sulkes J, Tambur A . Cytokine gene polymorphisms in patients infected with hepatitis B virus. Am J Gastroenterol. 2003; 98(1):144-50. DOI: 10.1111/j.1572-0241.2003.07179.x. View

Nemetz A, Molnar T, Kope A, Kovacs A, Feher J, Tulassay Z . IL1B gene polymorphisms influence the course and severity of inflammatory bowel disease. Immunogenetics. 1999; 49(6):527-31. DOI: 10.1007/s002510050530. View

10.

Garcia-Gonzalez M, Lanas A, Santolaria S, Crusius J, Serrano M, Pena A . The polymorphic IL-1B and IL-1RN genes in the aetiopathogenesis of peptic ulcer. Clin Exp Immunol. 2001; 125(3):368-75. PMC: 1906147. DOI: 10.1046/j.1365-2249.2001.01593.x. View

11.

Gabay C, Smith M, Eidlen D, Arend W . Interleukin 1 receptor antagonist (IL-1Ra) is an acute-phase protein. J Clin Invest. 1997; 99(12):2930-40. PMC: 508145. DOI: 10.1172/JCI119488. View

12.

Ma P, Chen D, Pan J, Du B . Genomic polymorphism within interleukin-1 family cytokines influences the outcome of septic patients. Crit Care Med. 2002; 30(5):1046-50. DOI: 10.1097/00003246-200205000-00015. View

13.

Kanemoto K, Kawasaki J, Miyamoto T, Obayashi H, Nishimura M . Interleukin (IL)1beta, IL-1alpha, and IL-1 receptor antagonist gene polymorphisms in patients with temporal lobe epilepsy. Ann Neurol. 2000; 47(5):571-4. View

14.

Santtila S, Savinainen K, Hurme M . Presence of the IL-1RA allele 2 (IL1RN*2) is associated with enhanced IL-1beta production in vitro. Scand J Immunol. 1998; 47(3):195-8. DOI: 10.1046/j.1365-3083.1998.00300.x. View

15.

Rapicetta M, Ferrari C, Levrero M . Viral determinants and host immune responses in the pathogenesis of HBV infection. J Med Virol. 2002; 67(3):454-7. DOI: 10.1002/jmv.10096. View

16.

Miyazoe S, Hamasaki K, Nakata K, Kajiya Y, Kitajima K, Nakao K . Influence of interleukin-10 gene promoter polymorphisms on disease progression in patients chronically infected with hepatitis B virus. Am J Gastroenterol. 2002; 97(8):2086-92. DOI: 10.1111/j.1572-0241.2002.05926.x. View

17.

Perrillo R . How will we use the new antiviral agents for hepatitis B?. Curr Gastroenterol Rep. 2002; 4(1):63-71. DOI: 10.1007/s11894-002-0039-6. View