» Articles » PMID: 15155803

Tom22', an 8-kDa Trans-site Receptor in Plants and Protozoans, is a Conserved Feature of the TOM Complex That Appeared Early in the Evolution of Eukaryotes

Overview
Journal Mol Biol Evol
Specialty Biology
Date 2004 May 25
PMID 15155803
Citations 36
Authors
Affiliations
Soon will be listed here.
Abstract

One of the earliest events in the evolution of mitochondria was the development a means to translocate proteins made in the cytosol into the "protomitochondrion." How this was achieved remains uncertain, and the nature of the earliest version of the protein translocation machinery is not known. Comparative sequence analysis suggests three subunits, Tom40, Tom7, and Tom22 as common elements of the protein translocase in the mitochondrial outer membrane in diverse extant eukaryotes. Tom22, the 22-kDa subunit, plays a critical role in the function of this complex in fungi and animals, and we show that an 8-kDa subunit of the plant translocase is a truncated form of Tom22. It has a single transmembrane segment conforming in sequence to the same region of Tom22 from other eukaryotic lineages and a short carboxy-terminal trans domain located in the mitochondrial intermembrane space. The trans domain from the Arabidopsis thaliana protein functions in yeast lacking their own Tom22 by complementing protein import defects and restoring cell growth. Moreover, we have identified orthologs of Tom22, Tom7, and Tom40 in diverse eukaryotes such as the diatom Phaeodactylum tricornutum, the amoebic slime Dictyostelium discoideum, and the protozoan parasite Plasmodium falciparum. This finding strongly suggests these subunits as the core of the protein translocase in the earliest mitochondria.

Citing Articles

Lessons from the deep: mechanisms behind diversification of eukaryotic protein complexes.

Prokopchuk G, Butenko A, Dacks J, Speijer D, Field M, Lukes J Biol Rev Camb Philos Soc. 2023; 98(6):1910-1927.

PMID: 37336550 PMC: 10952624. DOI: 10.1111/brv.12988.


The draft nuclear genome sequence and predicted mitochondrial proteome of Andalucia godoyi, a protist with the most gene-rich and bacteria-like mitochondrial genome.

Gray M, Burger G, Derelle R, Klimes V, Leger M, Sarrasin M BMC Biol. 2020; 18(1):22.

PMID: 32122349 PMC: 7050145. DOI: 10.1186/s12915-020-0741-6.


Homologue replacement in the import motor of the mitochondrial inner membrane of trypanosomes.

von Kanel C, Munoz-Gomez S, Oeljeklaus S, Wenger C, Warscheid B, Wideman J Elife. 2020; 9.

PMID: 32105215 PMC: 7064343. DOI: 10.7554/eLife.52560.


Structure of the mitochondrial import gate reveals distinct preprotein paths.

Araiso Y, Tsutsumi A, Qiu J, Imai K, Shiota T, Song J Nature. 2019; 575(7782):395-401.

PMID: 31600774 DOI: 10.1038/s41586-019-1680-7.


Recognition motifs rather than phylogenetic origin influence the ability of targeting peptides to import nuclear-encoded recombinant proteins into rice mitochondria.

Baysal C, Perez-Gonzalez A, Eseverri A, Jiang X, Medina V, Caro E Transgenic Res. 2019; 29(1):37-52.

PMID: 31598902 PMC: 7000509. DOI: 10.1007/s11248-019-00176-9.