Surface Plasmon Resonance Analysis of Antipolysaccharide Antibody Specificity: Responses to Meningococcal Group C Conjugate Vaccines and Bacteria

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2004 May 25

PMID 15155652

Citations 8

Authors

Pablo A Garcia-Ojeda

Sharon Hardy

Steven Kozlowski

Kathryn E Stein

Ian M Feavers

Affiliations

Soon will be listed here.

Abstract

Antibody (Ab) responses to polysaccharides (PS), such as Neisseria meningitidis group C PS (MCPS), are characterized as being thymus independent and are restricted with regard to clonotype and isotype expression. PS conjugated to proteins, e.g., MCPS coupled with tetanus toxoid or the diphtheria toxin derivative CRM197, elicit thymus-dependent responses. The present study developed a surface plasmon resonance approach to evaluate Ab responses to MCPS conjugate vaccines, including either O-acetylated (OAc+) or de-O-acetylated (OAc-) forms of the PS. The results were generally consistent with those obtained by enzyme-linked immunosorbent assay and showed that sera from mice immunized with conjugate vaccines contain Abs that bind more effectively to OAc+ and OAc- MCPS than sera from mice immunized with fixed bacteria. The data suggest a critical shared or overlapping epitope recognized by all the conjugate vaccine immune sera and strategies for assessing polyclonal Ab avidity.

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References

Adams W, Deaver K, Cochi S, Plikaytis B, Zell E, Broome C . Decline of childhood Haemophilus influenzae type b (Hib) disease in the Hib vaccine era. JAMA. 1993; 269(2):221-6. View

Twumasi Jr P, Kumah S, Leach A, ODempsey T, Ceesay S, Todd J . A trial of a group A plus group C meningococcal polysaccharide-protein conjugate vaccine in African infants. J Infect Dis. 1995; 171(3):632-8. DOI: 10.1093/infdis/171.3.632. View

Hutchinson A . Characterization of glycoprotein oligosaccharides using surface plasmon resonance. Anal Biochem. 1994; 220(2):303-7. DOI: 10.1006/abio.1994.1341. View

Cooper L, Robertson D, Granzow R, Greenspan N . Variable domain-identical antibodies exhibit IgG subclass-related differences in affinity and kinetic constants as determined by surface plasmon resonance. Mol Immunol. 1994; 31(8):577-84. DOI: 10.1016/0161-5890(94)90165-1. View

Borrow R, Longworth E, Gray S, Kaczmarski E . Prevalence of de-O-acetylated serogroup C meningococci before the introduction of meningococcal serogroup C conjugate vaccines in the United Kingdom. FEMS Immunol Med Microbiol. 2000; 28(3):189-91. DOI: 10.1111/j.1574-695X.2000.tb01475.x. View

. Impact of vaccines universally recommended for children--United States, 1990-1998. MMWR Morb Mortal Wkly Rep. 1999; 48(12):243-8. View

Borrow R, Southern J, Andrews N, Peake N, Rahim R, Acuna M . Comparison of antibody kinetics following meningococcal serogroup C conjugate vaccine between healthy adults previously vaccinated with meningococcal A/C polysaccharide vaccine and vaccine-naïve controls. Vaccine. 2001; 19(23-24):3043-50. DOI: 10.1016/s0264-410x(01)00050-0. View

Borrow R, Richmond P, Kaczmarski E, Iverson A, Martin S, Findlow J . Meningococcal serogroup C-specific IgG antibody responses and serum bactericidal titres in children following vaccination with a meningococcal A/C polysaccharide vaccine. FEMS Immunol Med Microbiol. 2000; 28(1):79-85. DOI: 10.1111/j.1574-695X.2000.tb01460.x. View

Ceesay S, Allen S, Menon A, TODD J, Cham K, Carlone G . Decline in meningococcal antibody levels in African children 5 years after vaccination and the lack of an effect of booster immunization. J Infect Dis. 1993; 167(5):1212-6. DOI: 10.1093/infdis/167.5.1212. View

10.

Rubinstein L, STEIN K . Murine immune response to the Neisseria meningitidis group C capsular polysaccharide. II. Specificity. J Immunol. 1988; 141(12):4357-62. View

11.

Peltola H, Kayhty H, Sivonen A, Makela H . Haemophilus influenzae type b capsular polysaccharide vaccine in children: a double-blind field study of 100,000 vaccinees 3 months to 5 years of age in Finland. Pediatrics. 1977; 60(5):730-7. View

12.

Goldschneider I, Gotschlich E, Artenstein M . Human immunity to the meningococcus. I. The role of humoral antibodies. J Exp Med. 1969; 129(6):1307-26. PMC: 2138650. DOI: 10.1084/jem.129.6.1307. View

13.

Mosier D, Zitron I, Mond J, Ahmed A, SCHER I, Paul W . Surface immunoglobulin D as a functional receptor for a subclass of B lymphocytes. Immunol Rev. 1977; 37:89-104. DOI: 10.1111/j.1600-065x.1977.tb00246.x. View

14.

Karlsson R, Michaelsson A, Mattsson L . Kinetic analysis of monoclonal antibody-antigen interactions with a new biosensor based analytical system. J Immunol Methods. 1991; 145(1-2):229-40. DOI: 10.1016/0022-1759(91)90331-9. View

15.

Joseph H, Miller E, Dawson M, Andrews N, Feavers I, Borrow R . Meningococcal serogroup a avidity indices as a surrogate marker of priming for the induction of immunologic memory after vaccination with a meningococcal A/C conjugate vaccine in infants in the United Kingdom. J Infect Dis. 2001; 184(5):661-2. DOI: 10.1086/322790. View

16.

Bhattacharjee A, Jennings H, KENNY C, Martin A, Smith I . Structural determination of the sialic acid polysaccharide antigens of Neisseria meningitidis serogroups B and C with carbon 13 nuclear magnetic resonance. J Biol Chem. 1975; 250(5):1926-32. View

17.

Beuvery E, Van Delft R, Miedema F, Kanhai V, Nagel J . Immunological evaluation of meningococcal group C polysaccharide-tetanus toxoid conjugate in mice. Infect Immun. 1983; 41(2):609-17. PMC: 264686. DOI: 10.1128/iai.41.2.609-617.1983. View

18.

Murphy T, White K, Pastor P, Gabriel L, Medley F, Granoff D . Declining incidence of Haemophilus influenzae type b disease since introduction of vaccination. JAMA. 1993; 269(2):246-8. View

19.

Andrews N, Borrow R, Miller E . Validation of serological correlate of protection for meningococcal C conjugate vaccine by using efficacy estimates from postlicensure surveillance in England. Clin Diagn Lab Immunol. 2003; 10(5):780-6. PMC: 193909. DOI: 10.1128/cdli.10.5.780-786.2003. View

20.

Ahlstedt S, Holmgren J, Hanson L . Protective capacity of antibodies against E. coli O antigen with special reference to the avidity. Int Arch Allergy Appl Immunol. 1974; 46(3):470-80. DOI: 10.1159/000231150. View