NHERF2 Specifically Interacts with LPA2 Receptor and Defines the Specificity and Efficiency of Receptor-mediated Phospholipase C-beta3 Activation

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 2004 May 15

PMID 15143197

Citations 46

Authors

Yong-Seok Oh

Nam Won Jo

Jung Woong Choi

Hyeon Soo Kim

Sang-Won Seo

Kyung-Ok Kang

Jong-Ik Hwang

Kyun Heo

Sun-Hee Kim

Yun-Hee Kim

In-Hoo Kim

Jae Ho Kim

Yoshiko Banno

Sung Ho Ryu

Pann-Ghill Suh

Affiliations

Soon will be listed here.

Abstract

Lysophosphatidic acid (LPA) activates a family of cognate G protein-coupled receptors and is involved in various pathophysiological processes. However, it is not clearly understood how these LPA receptors are specifically coupled to their downstream signaling molecules. This study found that LPA(2), but not the other LPA receptor isoforms, specifically interacts with Na(+)/H(+) exchanger regulatory factor2 (NHERF2). In addition, the interaction between them requires the C-terminal PDZ domain-binding motif of LPA(2) and the second PDZ domain of NHERF2. Moreover, the stable expression of NHERF2 potentiated LPA-induced phospholipase C-beta (PLC-beta) activation, which was markedly attenuated by either a mutation in the PDZ-binding motif of LPA(2) or by the gene silencing of NHERF2. Using its second PDZ domain, NHERF2 was found to indirectly link LPA(2) to PLC-beta3 to form a complex, and the other PLC-beta isozymes were not included in the protein complex. Consistently, LPA(2)-mediated PLC-beta activation was specifically inhibited by the gene silencing of PLC-beta3. In addition, NHERF2 increases LPA-induced ERK activation, which is followed by cyclooxygenase-2 induction via a PLC-dependent pathway. Overall, the results suggest that a ternary complex composed of LPA(2), NHERF2, and PLC-beta3 may play a key role in the LPA(2)-mediated PLC-beta signaling pathway.

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