Embryonic Expression of the Soma-restricted Products of the Myelin Proteolipid Gene in Motor Neurons and Muscle

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 2004 May 14

PMID 15139298

Citations 9

Authors

Erin C Jacobs

Ernesto R Bongarzone

Celia W Campagnoni

Anthony T Campagnoni

Affiliations

Soon will be listed here.

Abstract

In addition to classic proteolipid protein (PLP) and DM20, the mouse myelin proteolipid gene produces the sr-PLP and sr-DM20 proteins. The sr-isoforms are localized to the cell bodies of both oligodendrocytes and neurons. However, they are expressed to a greater extent in neurons than they are in glia. In this study, we examined expression of the sr-proteolipids in the mouse embryo using immunohistochemistry with an sr-PLP/DM20 specific antibody. Widespread expression of the sr-proteins was found in many nonmyelinating cell types. In particular, strong immunoreactivity was detected in motor neurons of both the autonomic and somatic nervous systems as well as in striated muscle. This pattern of expression persisted throughout the embryonic period studied. Thus, the sr-proteolipids are expressed prior to the onset of myelination and in a much broader array of cell types than their classic counterparts. These results support the conclusion that the sr-isoforms of the PLP gene have a biological role independent of myelination.

Citing Articles

The wmN1 Enhancer Region of the Mouse Myelin Proteolipid Protein Gene (mPlp1) is Indispensable for Expression of an mPlp1-lacZ Transgene in Both the CNS and PNS.

Patyal P, Kockara N, Wight P Neurochem Res. 2019; 45(3):663-671.

PMID: 31782102 PMC: 7060809. DOI: 10.1007/s11064-019-02919-w.

Transcriptome sequencing revealed significant alteration of cortical promoter usage and splicing in schizophrenia.

Wu J, Wang X, Beveridge N, Tooney P, Scott R, Carr V PLoS One. 2012; 7(4):e36351.

PMID: 22558445 PMC: 3338678. DOI: 10.1371/journal.pone.0036351.

Molecular genetic analysis of the PLP1 gene in 38 families with PLP1-related disorders: identification and functional characterization of 11 novel PLP1 mutations.

Grossi S, Regis S, Biancheri R, Mort M, Lualdi S, Bertini E Orphanet J Rare Dis. 2011; 6:40.

PMID: 21679407 PMC: 3125326. DOI: 10.1186/1750-1172-6-40.

The proteolipid protein promoter drives expression outside of the oligodendrocyte lineage during embryonic and early postnatal development.

Michalski J, Anderson C, Beauvais A, De Repentigny Y, Kothary R PLoS One. 2011; 6(5):e19772.

PMID: 21572962 PMC: 3091881. DOI: 10.1371/journal.pone.0019772.

The multiple roles of myelin protein genes during the development of the oligodendrocyte.

Fulton D, Paez P, Campagnoni A ASN Neuro. 2009; 2(1):e00027.

PMID: 20017732 PMC: 2814326. DOI: 10.1042/AN20090051.

References

Landry C, Ellison J, Skinner E, Campagnoni A . Golli-MBP proteins mark the earliest stages of fiber extension and terminal arboration in the mouse peripheral nervous system. J Neurosci Res. 1998; 50(2):265-71. DOI: 10.1002/(SICI)1097-4547(19971015)50:2<265::AID-JNR15>3.0.CO;2-7. View

Bongarzone E, Campagnoni C, Kampf K, JACOBS E, Handley V, Schonmann V . Identification of a new exon in the myelin proteolipid protein gene encoding novel protein isoforms that are restricted to the somata of oligodendrocytes and neurons. J Neurosci. 1999; 19(19):8349-57. PMC: 6783048. View

Landry C, Pribyl T, Ellison J, Givogri M, Kampf K, Campagnoni C . Embryonic expression of the myelin basic protein gene: identification of a promoter region that targets transgene expression to pioneer neurons. J Neurosci. 1998; 18(18):7315-27. PMC: 6793259. View

Nadon N, Miller S, Draeger K, Salvaggio M . Myelin proteolipid DM20: evidence for function independent of myelination. Int J Dev Neurosci. 1997; 15(3):285-93. DOI: 10.1016/s0736-5748(97)00009-9. View

Pribyl T, Campagnoni C, Kampf K, Ellison J, Landry C, Kashima T . Expression of the myelin basic protein gene locus in neurons and oligodendrocytes in the human fetal central nervous system. J Comp Neurol. 1996; 374(3):342-53. DOI: 10.1002/(SICI)1096-9861(19961021)374:3<342::AID-CNE3>3.0.CO;2-1. View

Pribyl T, Campagnoni C, Kampf K, Kashima T, Handley V, McMahon J . Expression of the myelin proteolipid protein gene in the human fetal thymus. J Neuroimmunol. 1996; 67(2):125-30. DOI: 10.1016/0165-5728(96)00058-6. View

Puckett C, Hudson L, Ono K, Friedrich V, Benecke J, Dubois-Dalcq M . Myelin-specific proteolipid protein is expressed in myelinating Schwann cells but is not incorporated into myelin sheaths. J Neurosci Res. 1987; 18(4):511-8. DOI: 10.1002/jnr.490180402. View

Timsit S, Bally-Cuif L, Colman D, Zalc B . DM-20 mRNA is expressed during the embryonic development of the nervous system of the mouse. J Neurochem. 1992; 58(3):1172-5. DOI: 10.1111/j.1471-4159.1992.tb09378.x. View

Campagnoni A, Skoff R . The pathobiology of myelin mutants reveal novel biological functions of the MBP and PLP genes. Brain Pathol. 2001; 11(1):74-91. PMC: 8098301. DOI: 10.1111/j.1750-3639.2001.tb00383.x. View

10.

Campagnoni C, Garbay B, Micevych P, Pribyl T, Kampf K, Handley V . DM20 mRNA splice product of the myelin proteolipid protein gene is expressed in the murine heart. J Neurosci Res. 1992; 33(1):148-55. DOI: 10.1002/jnr.490330119. View

11.

Jacobs E, Bongarzone E, Campagnoni C, Kampf K, Campagnoni A . Soma-restricted products of the myelin proteolipid gene are expressed primarily in neurons in the developing mouse nervous system. Dev Neurosci. 2003; 25(2-4):96-104. DOI: 10.1159/000072259. View

12.

Pribyl T, Campagnoni C, Kampf K, Handley V, Campagnoni A . The major myelin protein genes are expressed in the human thymus. J Neurosci Res. 1996; 45(6):812-9. DOI: 10.1002/(SICI)1097-4547(19960915)45:6<812::AID-JNR18>3.0.CO;2-X. View

13.

Landry C, Ellison J, Pribyl T, Campagnoni C, Kampf K, Campagnoni A . Myelin basic protein gene expression in neurons: developmental and regional changes in protein targeting within neuronal nuclei, cell bodies, and processes. J Neurosci. 1996; 16(8):2452-62. PMC: 6578761. View

14.

Bongarzone E, Jacobs E, Schonmann V, Campagnoni A . Classic and soma-restricted proteolipids are targeted to different subcellular compartments in oligodendrocytes. J Neurosci Res. 2001; 65(6):477-84. DOI: 10.1002/jnr.1177. View