TopBP1 Localises to Centrosomes in Mitosis and to Chromosome Cores in Meiosis

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 2004 May 13

PMID 15138768

Citations 22

Authors

Kaarina Reini

Lahja Uitto

David Perera

Peter B Moens

Raimundo Freire

Juhani E Syvaoja

Affiliations

Soon will be listed here.

Abstract

Topoisomerase IIbeta binding protein 1 (TopBP1), previously shown to localise to sites of DNA damage and to stalled replication forks, has been implicated in DNA replication and in DNA damage response. In this work we showed that TopBP1 was localised in structures other than stalled replication forks. In late mitosis TopBP1 localises to centrosomes in a manner similar to other DNA damage response proteins such as BRCA1 and p53. Spindle checkpoint activation does not affect this centrosomal localisation. Moreover, in the testis, we detected high levels of TopBP1 associated with meiotic prophase chromosome cores and the X-Y pair. Together, these data suggest a direct role of TopBP1 during both mitosis and meiotic prophase I.

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