Echinococcus Granulosus Strain Typing in Bulgaria: the G1 Genotype is Predominant in Intermediate and Definitive Wild Hosts

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2004 May 6

PMID 15127295

Citations 19

Authors

Isabel Breyer

Dimitrina Georgieva

Rossitza Kurdova

Bruno Gottstein

Affiliations

Soon will be listed here.

Abstract

Addressing the genetic variability in Echinococcus granulosus is epidemiologically important, as strain characteristics may influence the local transmission patterns of zoonotic cystic echinococcosis. To classify the genotype(s) present in intermediate (pig, cattle and sheep) and definitive (jackal and wolf) hosts in Bulgaria, a DNA-based approach was used to assess parasite protoscoleces or strobiles. Genes corresponding to coding and non-coding regions of the nuclear and mitochondrial genome ( ND-1, HBX, Act II, AgB-1) were amplified by PCR and subsequently sequenced. The sequences resolved were all found to be identical to those published for the common sheep strain of E. granulosus, indicating that the G1 genotype is predominant in Bulgaria. One microvariant for ND-1 was found in the pig isolates; however no epidemiological significance was attributed to this finding.

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References

Bowles J, McManus D . Rapid discrimination of Echinococcus species and strains using a polymerase chain reaction-based RFLP method. Mol Biochem Parasitol. 1993; 57(2):231-9. DOI: 10.1016/0166-6851(93)90199-8. View

Todorov T, Boeva V . Human echinococcosis in Bulgaria: a comparative epidemiological analysis. Bull World Health Organ. 1999; 77(2):110-8. PMC: 2557595. View

Bowles J, Blair D, McManus D . Molecular genetic characterization of the cervid strain ('northern form') of Echinococcus granulosus. Parasitology. 1994; 109 ( Pt 2):215-21. DOI: 10.1017/s0031182000076332. View

McManus D, Ding Z, Bowles J . A molecular genetic survey indicates the presence of a single, homogeneous strain of Echinococcus granulosus in north-western China. Acta Trop. 1994; 56(1):7-14. DOI: 10.1016/0001-706x(94)90035-3. View

Lymbery A . Interbreeding, monophyly and the genetic yardstick: species concepts in parasites. Parasitol Today. 1992; 8(6):208-11. DOI: 10.1016/0169-4758(92)90266-5. View

Eckert J, Thompson R, Lymbery A, Pawlowski Z, Gottstein B, Morgan U . Further evidence for the occurrence of a distinct strain of Echinococcus granulosus in European pigs. Parasitol Res. 1993; 79(1):42-8. DOI: 10.1007/BF00931216. View

Thompson R, Lymbery A, Constantine C . Variation in Echinococcus: towards a taxonomic revision of the genus. Adv Parasitol. 1995; 35:145-76. DOI: 10.1016/s0065-308x(08)60071-8. View

Reddy Y, Rao J, Butchaiah G, Sharma B . Random amplified polymorphic DNA for the specific detection of bubaline Echinococcus granulosus by hybridization assay. Vet Parasitol. 1998; 79(4):315-23. DOI: 10.1016/s0304-4017(98)00176-9. View

Silva C, Henrique , Ferreira B, Picon M, Gorfinkiel N, Ehrlich R . Molecular cloning and characterization of actin genes from Echinococcus granulosus. Mol Biochem Parasitol. 1993; 60(2):209-19. DOI: 10.1016/0166-6851(93)90132-h. View

10.

McManus D, Zhang W, Li J, Bartley P . Echinococcosis. Lancet. 2003; 362(9392):1295-304. DOI: 10.1016/S0140-6736(03)14573-4. View

11.

Haag K, Araujo A, Gottstein B, Siles-Lucas M, Thompson R, Zaha A . Breeding systems in Echinococcus granulosus (Cestoda; Taeniidae): selfing or outcrossing?. Parasitology. 1999; 118 ( Pt 1):63-71. DOI: 10.1017/s0031182098003485. View

12.

Eckert J, Conraths F, Tackmann K . Echinococcosis: an emerging or re-emerging zoonosis?. Int J Parasitol. 2000; 30(12-13):1283-94. DOI: 10.1016/s0020-7519(00)00130-2. View

13.

Snabel V, DAmelio S, Mathiopoulos K, Turcekova L, Dubinsky P . Molecular evidence for the presence of a G7 genotype of Echinococcus granulosus in Slovakia. J Helminthol. 2000; 74(2):177-81. View

14.

McManus D, Bowles J . Molecular genetic approaches to parasite identification: their value in diagnostic parasitology and systematics. Int J Parasitol. 1996; 26(7):687-704. DOI: 10.1016/0020-7519(96)82612-9. View

15.

Frosch P, Hartmann M, Muhlschlegel F, Frosch M . Sequence heterogeneity of the echinococcal antigen B. Mol Biochem Parasitol. 1994; 64(1):171-5. DOI: 10.1016/0166-6851(94)90145-7. View

16.

Pamilo P, Nei M . Relationships between gene trees and species trees. Mol Biol Evol. 1988; 5(5):568-83. DOI: 10.1093/oxfordjournals.molbev.a040517. View

17.

Lavikainen A, Lehtinen M, Meri T, Hirvela-Koski V, Meri S . Molecular genetic characterization of the Fennoscandian cervid strain, a new genotypic group (G10) of Echinococcus granulosus. Parasitology. 2003; 127(Pt 3):207-15. DOI: 10.1017/s0031182003003780. View

18.

Bowles J, Blair D, McManus D . Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol. 1992; 54(2):165-73. DOI: 10.1016/0166-6851(92)90109-w. View

19.

Thompson R, Lymbery A . The nature, extent and significance of variation within the genus Echinococcus. Adv Parasitol. 1988; 27:209-58. DOI: 10.1016/s0065-308x(08)60356-5. View