Determination of Minimal Regrowth Concentration (MRC) in Clinical Isolates of Various Biofilm-forming Bacteria

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 2004 Apr 30

PMID 15114870

Citations 13

Authors

L cernohorska

M Votava

Affiliations

Soon will be listed here.

Abstract

Based on the ability to attach to polymeric surfaces, the formation of biofilms was determined in 5 wild-type strains (Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumanii, Escherichia coli, Staphylococcus warneri). Using modified Christensen method, minimum regrowth concentration (MRC) of piperacillin, piperacillin-tazobactam, cefoperazon, ceftazidim, cefepim, meronem, ciprofloxacin, netilmicin and amikacin for Gram-negative and of ampicillin-sulbactam, chloramphenicol, tetracycline, clindamycin, vancomycin and teicoplanin for Gram-positive bacteria was estimated in trypticase-soy broth medium after a 1-d growth on polystyrene microtiter plates. Adherent bacterial populations exhibited reduced antimicrobial susceptibility, which was not shown in submerged cultures. Our results indicate that MRC can predict therapeutic outcome of antibiotic treatment better than the minimum inhibitory concentration tests commonly used.

Citing Articles

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Response of the Bacterial Community and Antibiotic Resistance in Overnight Stagnant Water from a Municipal Pipeline.

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Low-Frequency Ultrasound Enhances Bactericidal Activity of Antimicrobial Agents against Biofilm.

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as an Emerging Ubiquitous Pathogen: Looking Beyond Contemporary Antibiotic Therapy.

Adegoke A, Stenstrom T, Okoh A Front Microbiol. 2017; 8:2276.

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Effects of Fluoroquinolones and Azithromycin on Biofilm Formation of Stenotrophomonas maltophilia.

Wang A, Wang Q, Kudinha T, Xiao S, Zhuo C Sci Rep. 2016; 6:29701.

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