Characterization and Expression of U1snRNA Genes from Potato

Overview

Journal Plant Mol Biol

Date 1992 Sep 1

PMID 1511141

Citations 5

Authors

P Vaux

F Guerineau

R Waugh

J W Brown

Affiliations

Soon will be listed here.

Abstract

U1 small nuclear RNAs (U1snRNAs) occur in the nucleus of plants and animals where, complexed with several proteins in the form of U1 small nuclear ribonucleoprotein particles (U1snRNPs), they play an important role in precursor messenger RNA (pre-mRNA) splicing. Ten potato U1snRNA genes have been isolated on two genomic clones illustrating the clustering of this multigene family on the potato genome. Based on both the sequence of their coding regions and upstream regulatory elements, seven of the genes are potentially functional. The other three genes were pseudogenes with defective promoter or coding region sequences. Analysis of expression of individual cloned U1snRNA genes in transfected tobacco protoplasts was impossible due to the similarity of U1snRNA sequences in tobacco. However, by marking the coding regions with oligonucleotides or constructing chimaeric genes consisting of a potato U1snRNA promoter region and maize U5snRNA coding region, three of the U1 promoter regions were shown to be transcriptionally active.

Citing Articles

Analysis of RNA silencing in agroinfiltrated leaves of Nicotiana benthamiana and Nicotiana tabacum.

Koscianska E, Kalantidis K, Wypijewski K, Sadowski J, Tabler M Plant Mol Biol. 2005; 59(4):647-61.

PMID: 16244913 DOI: 10.1007/s11103-005-0668-x.

Structure and expression of a plant U1 snRNP 70K gene: alternative splicing of U1 snRNP 70K pre-mRNAs produces two different transcripts.

Golovkin M, Reddy A Plant Cell. 1996; 8(8):1421-35.

PMID: 8776903 PMC: 161266. DOI: 10.1105/tpc.8.8.1421.

Differential expression of U5snRNA gene variants in maize (Zea mays) protoplasts.

Leader D, Connelly S, Filipowicz W, Waugh R, Brown J Plant Mol Biol. 1993; 21(1):133-43.

PMID: 8425043 DOI: 10.1007/BF00039624.

The U6 small nuclear RNA gene family of potato.

Guerineau F, Waugh R Plant Mol Biol. 1993; 22(5):807-18.

PMID: 8358031 DOI: 10.1007/BF00027367.

Expression analysis of multigene families by RT-PCR.

Simpson C, Sawbridge T, Jenkins G, Brown J Nucleic Acids Res. 1992; 20(21):5861-2.

PMID: 1280813 PMC: 334444. DOI: 10.1093/nar/20.21.5861.

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