Factors Affecting the Rate of T-DNA Transfer from Agrobacterium Tumefaciens to Nicotiana Glauca Plant Cells

Overview

Journal Plant Mol Biol

Date 1992 Sep 1

PMID 1511127

Citations 10

Authors

T Mozo

P J Hooykaas

Affiliations

Soon will be listed here.

Abstract

Different factors involved in the early steps of the T-DNA transfer process were studied by using a beta-glucuronidase gene (gusA) as a reporter in Nicotiana glauca leaf disc transformation experiments. The levels of transient expression of the gusA gene in leaf discs infected with several strains or vir mutants correlated well with their virulence phenotype, except for virC mutants. The rate of T-DNA transfer was shown to be stimulated in the case of non-oncogenic strains by the co-transfer of small amounts of oncogenic genes. It was found that the location of the T-DNA in the Agrobacterium genome affected the T-DNA transfer rate especially in virC mutants. The virC mutants transferred the gusA-containing T-DNA located on a binary vector more efficiently than the oncogenic T-DNA of the Ti plasmid. Although wild-type strains induced high levels of gusA expression early after infection, the gusA expression appeared to be lost late after infection in the infected leaf discs. In contrast, in leaf discs infected by virC mutants the level of gusA expression increased steadily in time. A model explaining these results is presented.

Citing Articles

Agrobacterium-mediated transformation of Aspergillus awamori in the absence of full-length VirD2, VirC2, or VirE2 leads to insertion of aberrant T-DNA structures.

Michielse C, Ram A, Hooykaas P, Van Den Hondel C J Bacteriol. 2004; 186(7):2038-45.

PMID: 15028687 PMC: 374399. DOI: 10.1128/JB.186.7.2038-2045.2004.

Ectopic expression of a c1-I allele from maize inhibits pigment formation in the flower of transgenic tobacco.

Chen B, Wang X, Hu Y, Wang Y, Lin Z Mol Biotechnol. 2004; 26(3):187-92.

PMID: 15004287 DOI: 10.1385/MB:26:3:187.

Shoot production per responsive leaf explant increases exponentially with explant organogenic potential in Nicotiana species.

Li B, Huang W, Bass T Plant Cell Rep. 2003; 22(4):231-8.

PMID: 12920564 DOI: 10.1007/s00299-003-0679-5.

Promoter of the rolC gene of Agrobacterium rhizogenes can be strongly regulated in glandular cell of transgenic tobacco.

Hu Y, Chen B, Ni T, Li N, Lin Z Mol Biotechnol. 2003; 24(2):121-6.

PMID: 12746553 DOI: 10.1385/MB:24:2:121.

Purine synthesis and increased Agrobacterium tumefaciens transformation of yeast and plants.

Roberts R, Metz M, Monks D, Mullaney M, Hall T, Nester E Proc Natl Acad Sci U S A. 2003; 100(11):6634-9.

PMID: 12740435 PMC: 164499. DOI: 10.1073/pnas.1132022100.

References

Thompson D, Melchers L, Idler K, Schilperoort R, Hooykaas P . Analysis of the complete nucleotide sequence of the Agrobacterium tumefaciens virB operon. Nucleic Acids Res. 1988; 16(10):4621-36. PMC: 336653. DOI: 10.1093/nar/16.10.4621. View

Melchers L, Maroney M, Thompson D, van Vuuren H, Schilperoort R, Hooykaas P . Octopine and nopaline strains of Agrobacterium tumefaciens differ in virulence; molecular characterization of the virF locus. Plant Mol Biol. 1990; 14(2):249-59. DOI: 10.1007/BF00018565. View

Ditta G, Stanfield S, Corbin D, Helinski D . Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980; 77(12):7347-51. PMC: 350500. DOI: 10.1073/pnas.77.12.7347. View

Stachel S, Zambryski P . virA and virG control the plant-induced activation of the T-DNA transfer process of A. tumefaciens. Cell. 1986; 46(3):325-33. DOI: 10.1016/0092-8674(86)90653-7. View

Schroder G, Klipp W, Hillebrand A, Ehring R, Koncz C, Schroder J . The conserved part of the T-region in Ti-plasmids expresses four proteins in bacteria. EMBO J. 1983; 2(3):403-9. PMC: 555147. DOI: 10.1002/j.1460-2075.1983.tb01437.x. View

Hobbs S, Kpodar P, Delong C . The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants. Plant Mol Biol. 1990; 15(6):851-64. DOI: 10.1007/BF00039425. View

Zambryski P, Joos H, Genetello C, Leemans J, Montagu M, Schell J . Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity. EMBO J. 1983; 2(12):2143-50. PMC: 555426. DOI: 10.1002/j.1460-2075.1983.tb01715.x. View

Hirsch P, Beringer J . A physical map of pPH1JI and pJB4JI. Plasmid. 1984; 12(2):139-41. DOI: 10.1016/0147-619x(84)90059-3. View

Horsch R, Klee H, Stachel S, Winans S, Nester E, Rogers S . Analysis of Agrobacterium tumefaciens virulence mutants in leaf discs. Proc Natl Acad Sci U S A. 1986; 83(8):2571-5. PMC: 323340. DOI: 10.1073/pnas.83.8.2571. View

10.

Napoli C, Lemieux C, Jorgensen R . Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans. Plant Cell. 1990; 2(4):279-289. PMC: 159885. DOI: 10.1105/tpc.2.4.279. View

11.

Stachel S, Nester E . The genetic and transcriptional organization of the vir region of the A6 Ti plasmid of Agrobacterium tumefaciens. EMBO J. 1986; 5(7):1445-54. PMC: 1166964. DOI: 10.1002/j.1460-2075.1986.tb04381.x. View

12.

Durrenberger F, Crameri A, Hohn B . Covalently bound VirD2 protein of Agrobacterium tumefaciens protects the T-DNA from exonucleolytic degradation. Proc Natl Acad Sci U S A. 1989; 86(23):9154-8. PMC: 298452. DOI: 10.1073/pnas.86.23.9154. View

13.

van Haaren M, Sedee N, Schilperoort R, Hooykaas P . Overdrive is a T-region transfer enhancer which stimulates T-strand production in Agrobacterium tumefaciens. Nucleic Acids Res. 1987; 15(21):8983-97. PMC: 306417. DOI: 10.1093/nar/15.21.8983. View

14.

van der Krol A, Mur L, Beld M, Mol J, Stuitje A . Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression. Plant Cell. 1990; 2(4):291-9. PMC: 159886. DOI: 10.1105/tpc.2.4.291. View

15.

Timmerman B, Van Montagu M, Zambryski P . vir-induced recombination in Agrobacterium. Physical characterization of precise and imprecise T-circle formation. J Mol Biol. 1988; 203(2):373-84. DOI: 10.1016/0022-2836(88)90005-8. View

16.

Toro N, Datta A, Carmi O, Young C, Prusti R, Nester E . The Agrobacterium tumefaciens virC1 gene product binds to overdrive, a T-DNA transfer enhancer. J Bacteriol. 1989; 171(12):6845-9. PMC: 210585. DOI: 10.1128/jb.171.12.6845-6849.1989. View

17.

Lulsdorf M, Rempel H, Jackson J, Baliski D, Hobbs S . Optimizing the production of transformed pea (Pisum sativum L.) callus using disarmed Agrobacterium tumefaciens strains. Plant Cell Rep. 2013; 9(9):479-83. DOI: 10.1007/BF00232100. View

18.

Citovsky V, Wong M, Zambryski P . Cooperative interaction of Agrobacterium VirE2 protein with single-stranded DNA: implications for the T-DNA transfer process. Proc Natl Acad Sci U S A. 1989; 86(4):1193-7. PMC: 286652. DOI: 10.1073/pnas.86.4.1193. View

19.

Mozo T, Hooykaas P . Electroporation of megaplasmids into Agrobacterium. Plant Mol Biol. 1991; 16(5):917-8. DOI: 10.1007/BF00015085. View

20.

Mittelsten Scheid O, Paszkowski J, Potrykus I . Reversible inactivation of a transgene in Arabidopsis thaliana. Mol Gen Genet. 1991; 228(1-2):104-12. DOI: 10.1007/BF00282454. View