Immunofluorescence Assay for Serologic Diagnosis of SARS

Overview

Journal Emerg Infect Dis

Specialty Infectious Diseases

Date 2004 Apr 28

PMID 15109430

Citations 42

Authors

Paul K S Chan

King-Cheung Ng

Rickjason C W Chan

Rebecca K Y Lam

Viola C Y Chow

Mamie Hui

Alan Wu

Nelson Lee

Florence H Y Yap

Frankie W T Cheng

Joseph J Y Sung

John S Tam

Affiliations

Soon will be listed here.

Abstract

We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.

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