Adaptive Cytoprotection Induced by Pretreatment with Ethanol Protects Against Gastric Cell Damage by NSAIDs

Overview

Journal Dig Dis Sci

Specialty Gastroenterology

Date 2004 Apr 24

PMID 15104359

Citations 5

Authors

Ken-Ichiro Tanaka

Kiyo Nishimoto

Wataru Tomisato

Shinji Tsutsumi

Tatsuya Hoshino

Tomofusa Tsuchiya

Tohru Mizushima

Affiliations

Soon will be listed here.

Abstract

In this study, we examined adaptive cytoprotection against NSAIDs in human gastric carcinoma cells in culture. Pretreatment of cells with low (nontoxic) concentrations of ethanol protected cells from cell death induced by subsequent exposure to NSAIDs. The adaptive cytoprotection against NSAIDs induced by ethanol was not attenuated by pretreatment of cells with inhibitors of protein synthesis or prostaglandin synthesis, thus inferring that neither newly synthesized proteins nor prostaglandins are involved in this process. Furthermore, treatment of cells with the low concentration of ethanol did not affect the synthesis and secretion of mucin. In in vivo experiments on rats, oral preadministration of a low dose of ethanol protected the gastric mucosa from gastric lesions induced by subsequent oral administration of NSAIDs. One possible explanation for this in vivo phenomenon is that the adaptive cytoprotection induced by ethanol protects the gastric mucosa from the direct cytotoxic effect of NSAIDs.

Citing Articles

Acetaldehyde is an oxidative stressor for gastric epithelial cells.

Tamura M, Ito H, Matsui H, Hyodo I J Clin Biochem Nutr. 2014; 55(1):26-31.

PMID: 25120276 PMC: 4078068. DOI: 10.3164/jcbn.14-12.

Alcohol is an oxidative stressor for gastric epithelial cells: detection of superoxide in living cells.

Tamura M, Matsui H, Kaneko T, Hyodo I J Clin Biochem Nutr. 2013; 53(2):75-80.

PMID: 24062603 PMC: 3774929. DOI: 10.3164/jcbn.13-32.

The anti-apoptosis protein, survivin, mediates gastric epithelial cell cytoprotection against ethanol-induced injury via activation of the p34(cdc2) cyclin-dependent kinase.

Jones M, Padilla O, Webb N, Norng M J Cell Physiol. 2008; 215(3):750-64.

PMID: 18181150 PMC: 2288653. DOI: 10.1002/jcp.21358.

Effect of different cyclooxygenase inhibitors on gastric adaptive cytoprotection induced by 20% ethanol.

Gambero A, Marostica M, Becker T, Pedrazzoli Jr J Dig Dis Sci. 2007; 52(2):425-33.

PMID: 17226071 DOI: 10.1007/s10620-006-9487-4.

Case-control study on the association of upper gastrointestinal bleeding and nonsteroidal anti-inflammatory drugs in Japan.

Sakamoto C, Sugano K, Ota S, Sakaki N, Takahashi S, Yoshida Y Eur J Clin Pharmacol. 2006; 62(9):765-72.

PMID: 16821007 DOI: 10.1007/s00228-006-0171-6.

References

Tomisato W, Tsutsumi S, Tsuchiya T, Mizushima T . Geranylgeranylacetone protects guinea pig gastric mucosal cells from gastric stressor-induced necrosis by induction of heat-shock proteins. Biol Pharm Bull. 2001; 24(8):887-91. DOI: 10.1248/bpb.24.887. View

Tomisato W, Takahashi N, Komoto C, Rokutan K, Tsuchiya T, Mizushima T . Geranylgeranylacetone protects cultured guinea pig gastric mucosal cells from indomethacin. Dig Dis Sci. 2000; 45(8):1674-9. DOI: 10.1023/a:1005597902470. View

Gabriel S, Jaakkimainen L, Bombardier C . Risk for serious gastrointestinal complications related to use of nonsteroidal anti-inflammatory drugs. A meta-analysis. Ann Intern Med. 1991; 115(10):787-96. DOI: 10.7326/0003-4819-115-10-787. View

Miller T . Protective effects of prostaglandins against gastric mucosal damage: current knowledge and proposed mechanisms. Am J Physiol. 1983; 245(5 Pt 1):G601-23. DOI: 10.1152/ajpgi.1983.245.5.G601. View

Tsutsumi S, Mima S, Tomisato W, Hoshino T, Tsuchiya T, Mizushima T . Molecular mechanism of adaptive cytoprotection induced by ethanol in human gastric cells. Exp Biol Med (Maywood). 2003; 228(9):1089-95. DOI: 10.1177/153537020322800917. View

Kokoska E, Smith G, Wolff A, Deshpande Y, Rieckenberg C, Banan A . Role of calcium in adaptive cytoprotection and cell injury induced by deoxycholate in human gastric cells. Am J Physiol. 1998; 275(2):G322-30. DOI: 10.1152/ajpgi.1998.275.2.G322. View

Nakamura K, Rokutan K, Marui N, AOIKE A, Kawai K . Induction of heat shock proteins and their implication in protection against ethanol-induced damage in cultured guinea pig gastric mucosal cells. Gastroenterology. 1991; 101(1):161-6. DOI: 10.1016/0016-5085(91)90473-x. View

Watanabe T, OHara S, Miyazawa S, Saigenji K, Hotta K . Augmentative effects of L-cysteine and methylmethionine sulfonium chloride on mucin secretion in rabbit gastric mucous cells. J Gastroenterol Hepatol. 2000; 15(1):45-52. DOI: 10.1046/j.1440-1746.2000.02037.x. View

Glavin G, Lockhart L, Rockman G, Hall A, Kiernan K . Evidence of "cross-stressor"-induced adaptive gastric cytoprotection. Life Sci. 1987; 41(19):2223-7. DOI: 10.1016/0024-3205(87)90519-4. View

10.

Robert A, NEZAMIS J, Lancaster C, Davis J, Field S, Hanchar A . Mild irritants prevent gastric necrosis through "adaptive cytoprotection" mediated by prostaglandins. Am J Physiol. 1983; 245(1):G113-21. DOI: 10.1152/ajpgi.1983.245.1.G113. View

11.

Ligumsky M, Sestieri M, Karmeli F, Rachmilewitz D . Protection by mild irritants against indomethacin-induced gastric mucosal damage in the rat: role of prostaglandin synthesis. Isr J Med Sci. 1986; 22(11):807-11. View

12.

Kokoska E, Smith G, Deshpande Y, Rieckenberg C, Miller T . Adaptive cytoprotection induced by ethanol in human intestinal cells: role of prostaglandins and calcium homeostasis. Ann Surg. 1998; 228(1):123-30. PMC: 1191437. DOI: 10.1097/00000658-199807000-00018. View

13.

Singh G . Recent considerations in nonsteroidal anti-inflammatory drug gastropathy. Am J Med. 1998; 105(1B):31S-38S. DOI: 10.1016/s0002-9343(98)00072-2. View

14.

Takano T, Tsutsumi S, Tomisato W, Hoshino T, Tsuchiya T, Mizushima T . Geranylgeranylacetone protects guinea pig gastric mucosal cells from gastric stressor-induced apoptosis. Dig Dis Sci. 2002; 47(7):1546-53. DOI: 10.1023/a:1015819203084. View

15.

Kauffman Jr G, Reeve Jr J, GROSSMAN M . Gastric bicarbonate secretion: effect of topical and intravenous 16,16-dimethyl prostaglandin E2. Am J Physiol. 1980; 239(1):G44-8. DOI: 10.1152/ajpgi.1980.239.1.G44. View

16.

Basque J, Chenard M, Chailler P, Menard D . Gastric cancer cell lines as models to study human digestive functions. J Cell Biochem. 2001; 81(2):241-51. View

17.

Hoshino T, Takano T, Tsutsumi S, Tomisato W, Tsuchiya T, Mizushima T . Effects of prostaglandin E2 on gastric irritant-induced apoptosis. Dig Dis Sci. 2002; 47(10):2370-9. DOI: 10.1023/a:1020164000898. View

18.

Kurata J, Abbey D . The effect of chronic aspirin use on duodenal and gastric ulcer hospitalizations. J Clin Gastroenterol. 1990; 12(3):260-6. DOI: 10.1097/00004836-199006000-00005. View

19.

Wolf B, Green D . Suicidal tendencies: apoptotic cell death by caspase family proteinases. J Biol Chem. 1999; 274(29):20049-52. DOI: 10.1074/jbc.274.29.20049. View

20.

OHara S, Watanabe T, Hotta K . Comparative study of carbohydrate portion of gastrointestinal mucins using enzyme-linked lectin-binding assay (ELLA). Comp Biochem Physiol B Biochem Mol Biol. 1997; 116(2):167-72. DOI: 10.1016/s0305-0491(96)00218-0. View