Isolation and Characterization of the Chicken Bcl-2 Gene: Expression in a Variety of Tissues Including Lymphoid and Neuronal Organs in Adult and Embryo

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1992 Aug 25

PMID 1508712

Citations 20

Authors

Y Eguchi

D L Ewert

Y Tsujimoto

Affiliations

Soon will be listed here.

Abstract

The expression of human bcl-2 gene is de-regulated by t(14;18) translocation in most of follicular lymphoma. Recent studies indicated that the bcl-2 gene product has an ability to block apoptosis of hematopoietic cells. To facilitate the analysis of the role of this gene in normal development using an animal model, we have isolated and partially characterized the chicken homologue of human bcl-2 gene. The analysis of nucleotide sequence showed that the organization of the chicken bcl-2 gene is very similar to that of human bcl-2 gene. The primary transcript is spliced to encode a 25,687 dalton (233 a.a.) protein. The chicken Bcl-2 protein has two regions highly homologous to human Bcl-2 protein surrounding a totally non-homologous region. The expression of the chicken bcl-2 gene was analyzed in various chicken tissues. In the adult chicken, bcl-2 transcripts were detected in thymus, spleen, kidney, heart, ovary and brain, with the highest levels being detected in the thymus. However, the bursa of Fabricius, which is the site of early B cell development, expressed much less amounts of bcl-2 RNA. On the other hand, in embryo, the gene is extensively expressed in the bursa, as well as in muscle and the above tissues. Our findings indicate that a homologue of the human bcl-2 gene does exist in the chicken and that its expression is developmentally regulated in some tissues.

Citing Articles

The pathobiology of follicular lymphoma.

Carreras J J Clin Exp Hematop. 2023; 63(3):152-163.

PMID: 37518274 PMC: 10628832. DOI: 10.3960/jslrt.23014.

Specific gene expression signatures of low grade meningiomas.

Tsitsikov E, Hameed S, Tavakol S, Stephens T, Tsytsykova A, Garman L Front Oncol. 2023; 13:1126550.

PMID: 36937440 PMC: 10016690. DOI: 10.3389/fonc.2023.1126550.

AZD6738 Inhibits fibrotic response of conjunctival fibroblasts by regulating checkpoint kinase 1/ and / pathways.

Huang L, Ye Q, Lan C, Wang X, Zhu Y Front Pharmacol. 2022; 13:990401.

PMID: 36204234 PMC: 9530343. DOI: 10.3389/fphar.2022.990401.

Bioinformatic identification and expression analysis of the chicken B cell lymphoma (BCL) gene.

Than V, Tran H, Ly D, Dang H, Nguyen M, Truong A Genes Genomics. 2019; 41(10):1195-1206.

PMID: 31313104 DOI: 10.1007/s13258-019-00849-z.

Pro-Nifuroxazide Self-Assembly Leads to Triggerable Nanomedicine for Anti-cancer Therapy.

Misra S, Wu Z, Ostadhossein F, Ye M, Boateng K, Schulten K ACS Appl Mater Interfaces. 2019; 11(20):18074-18089.

PMID: 31013055 PMC: 7066988. DOI: 10.1021/acsami.9b01343.

References

Vieira J, Messing J . Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985; 33(1):103-19. DOI: 10.1016/0378-1119(85)90120-9. View

Padgett R, Grabowski P, Konarska M, Seiler S, Sharp P . Splicing of messenger RNA precursors. Annu Rev Biochem. 1986; 55:1119-50. DOI: 10.1146/annurev.bi.55.070186.005351. View

REYNAUD C, Anquez V, Grimal H, Weill J . A hyperconversion mechanism generates the chicken light chain preimmune repertoire. Cell. 1987; 48(3):379-88. DOI: 10.1016/0092-8674(87)90189-9. View

Vaux D, Cory S, Adams J . Bcl-2 gene promotes haemopoietic cell survival and cooperates with c-myc to immortalize pre-B cells. Nature. 1988; 335(6189):440-2. DOI: 10.1038/335440a0. View

Tsujimoto Y, Ikegaki N, Croce C . Characterization of the protein product of bcl-2, the gene involved in human follicular lymphoma. Oncogene. 1987; 2(1):3-7. View

Tsujimoto Y, Croce C . Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. Proc Natl Acad Sci U S A. 1986; 83(14):5214-8. PMC: 323921. DOI: 10.1073/pnas.83.14.5214. View

Tsujimoto Y, Cossman J, Jaffe E, Croce C . Involvement of the bcl-2 gene in human follicular lymphoma. Science. 1985; 228(4706):1440-3. DOI: 10.1126/science.3874430. View

REED R, Maniatis T . Intron sequences involved in lariat formation during pre-mRNA splicing. Cell. 1985; 41(1):95-105. DOI: 10.1016/0092-8674(85)90064-9. View

Bakhshi A, Jensen J, Goldman P, Wright J, MCBRIDE O, Epstein A . Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18. Cell. 1985; 41(3):899-906. DOI: 10.1016/s0092-8674(85)80070-2. View

10.

TOIVANEN P, Toivanen A . Bursal and postbursal stem cells in chicken. Functional characteristics. Eur J Immunol. 1973; 3(9):585-95. DOI: 10.1002/eji.1830030912. View

11.

Ivanyi J . Sequential recruitment of antibody class-committed B lymphocytes during ontogeny. Eur J Immunol. 1973; 3(12):789-93. DOI: 10.1002/eji.1830031210. View

12.

Dugaiczyk A, Haron J, Stone E, Dennison O, Rothblum K, Schwartz R . Cloning and sequencing of a deoxyribonucleic acid copy of glyceraldehyde-3-phosphate dehydrogenase messenger ribonucleic acid isolated from chicken muscle. Biochemistry. 1983; 22(7):1605-13. DOI: 10.1021/bi00276a013. View

13.

Frischauf A, Lehrach H, Poustka A, Murray N . Lambda replacement vectors carrying polylinker sequences. J Mol Biol. 1983; 170(4):827-42. DOI: 10.1016/s0022-2836(83)80190-9. View

14.

Jalkanen S, Jalkanen M, Granfors K, TOIVANEN P . Defect in the generation of light-chain diversity in bursectomized chickens. Nature. 1984; 311(5981):69-71. DOI: 10.1038/311069a0. View

15.

Mount S . A catalogue of splice junction sequences. Nucleic Acids Res. 1982; 10(2):459-72. PMC: 326150. DOI: 10.1093/nar/10.2.459. View

16.

Huang H, Dreyer W . Bursectomy in ovo blocks the generation of immunoglobulin diversity. J Immunol. 1978; 121(5):1738-47. View

17.

Maxam A, Gilbert W . A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977; 74(2):560-4. PMC: 392330. DOI: 10.1073/pnas.74.2.560. View

18.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

19.

Le Douarin N, Houssaint E, Jotereau F, Belo M . Origin of hemopoietic stem cells in embryonic bursa of Fabricius and bone marrow studied through interspecific chimeras. Proc Natl Acad Sci U S A. 1975; 72(7):2701-5. PMC: 432838. DOI: 10.1073/pnas.72.7.2701. View

20.

Liu Y, Mason D, JOHNSON G, Abbot S, Gregory C, Hardie D . Germinal center cells express bcl-2 protein after activation by signals which prevent their entry into apoptosis. Eur J Immunol. 1991; 21(8):1905-10. DOI: 10.1002/eji.1830210819. View