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Low-power High-resolution Solid-state NMR of Peptides and Proteins

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Journal J Am Chem Soc
Specialty Chemistry
Date 2004 Apr 15
PMID 15080665
Citations 18
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Abstract

We demonstrate that two-dimensional solid-state NMR chemical-shift correlation spectra can be recorded under low-power conditions. Except for the cross-polarization period, no rf-field amplitudes above 40 kHz are used. Such experiments require the use of fast (>50 kHz) magic-angle spinning (MAS). A comparison with the high-power version of the experiment shows no general line broadening but some changes in the polarization-transfer dynamics.

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