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Evaluation of PorB PCR-amplicon Restriction Endonuclease Analysis As a Method to Determine PorB Variable-region Sequences in Nonserotypeable Meningococci

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 2004 Apr 9
PMID 15071034
Citations 3
Authors
Kristin H Dyet
Robin S Simmonds
Diana R Martin
Affiliations
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Abstract

porB PCR-amplicon restriction endonuclease analysis is a rapid, simple method developed to assess porB variation in nonserotypeable meningococci isolated during New Zealand's epidemic of meningococcal disease. Most nonserotypeable meningococci isolated between 1990 and 1999 inclusively either were type 4 (40.5%) or contained the porB variable region 1 (VR1)-19, VR2-D, VR3-7, and VR4-14a sequences (45.1%).

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