PhoP Can Activate Its Target Genes in a PhoQ-independent Manner

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2004 Apr 3

PMID 15060051

Citations 36

Authors

Sergio Lejona

Maria Eugenia Castelli

Maria Laura Cabeza

Linda J Kenney

Eleonora Garcia Vescovi

Fernando C Soncini

Affiliations

Soon will be listed here.

Abstract

The PhoP/PhoQ two-component system controls the extracellular magnesium depletion response in Salmonella enterica. Previous studies have shown that PhoP is unable to up-regulate its target genes in the absence of PhoQ function. In this work, we demonstrate that PhoP overexpression can substitute for PhoQ- and phosphorylation-dependent activation. Either a high concentration of PhoP or activation via phosphorylation stimulates PhoP self-association.

Citing Articles

An E. coli display method for characterization of peptide-sensor kinase interactions.

Brink K, Hunt M, Mu A, Groszman K, Hoang K, Lorch K Nat Chem Biol. 2022; 19(4):451-459.

PMID: 36482094 PMC: 10065900. DOI: 10.1038/s41589-022-01207-z.

Coordination of Phosphate and Magnesium Metabolism in Bacteria.

Bruna R, Kendra C, Pontes M Adv Exp Med Biol. 2022; 1362:135-150.

PMID: 35288878 DOI: 10.1007/978-3-030-91623-7_12.

Methylation of PhoP by CheR Regulates Virulence.

Su Y, Li J, Zhang W, Ni J, Huang R, Wang Z mBio. 2021; 12(5):e0209921.

PMID: 34544273 PMC: 8546571. DOI: 10.1128/mBio.02099-21.

How the PhoP/PhoQ System Controls Virulence and Mg Homeostasis: Lessons in Signal Transduction, Pathogenesis, Physiology, and Evolution.

Groisman E, Duprey A, Choi J Microbiol Mol Biol Rev. 2021; 85(3):e0017620.

PMID: 34191587 PMC: 8483708. DOI: 10.1128/MMBR.00176-20.

DNA-Binding and Transcription Activation by Unphosphorylated Response Regulator AgrR From Involved in Silver Resistance.

Ali M, Provoost A, Mijnendonckx K, Van Houdt R, Charlier D Front Microbiol. 2020; 11:1635.

PMID: 32765465 PMC: 7380067. DOI: 10.3389/fmicb.2020.01635.

References

Da Re S, Schumacher J, Rousseau P, Fourment J, Ebel C, Kahn D . Phosphorylation-induced dimerization of the FixJ receiver domain. Mol Microbiol. 1999; 34(3):504-11. DOI: 10.1046/j.1365-2958.1999.01614.x. View

Head C, TARDY A, Kenney L . Relative binding affinities of OmpR and OmpR-phosphate at the ompF and ompC regulatory sites. J Mol Biol. 1998; 281(5):857-70. DOI: 10.1006/jmbi.1998.1985. View

Aguirre A, Lejona S, Vescovi E, Soncini F . Phosphorylated PmrA interacts with the promoter region of ugd in Salmonella enterica serovar typhimurium. J Bacteriol. 2000; 182(13):3874-6. PMC: 94567. DOI: 10.1128/JB.182.13.3874-3876.2000. View

Castelli M, Garcia Vescovi E, Soncini F . The phosphatase activity is the target for Mg2+ regulation of the sensor protein PhoQ in Salmonella. J Biol Chem. 2000; 275(30):22948-54. DOI: 10.1074/jbc.M909335199. View

Lee J, Owens J, Hwang I, Meares C, Kustu S . Phosphorylation-induced signal propagation in the response regulator ntrC. J Bacteriol. 2000; 182(18):5188-95. PMC: 94668. DOI: 10.1128/JB.182.18.5188-5195.2000. View

Ellison D, McCleary W . The unphosphorylated receiver domain of PhoB silences the activity of its output domain. J Bacteriol. 2000; 182(23):6592-7. PMC: 111398. DOI: 10.1128/JB.182.23.6592-6597.2000. View

Groisman E . The pleiotropic two-component regulatory system PhoP-PhoQ. J Bacteriol. 2001; 183(6):1835-42. PMC: 95077. DOI: 10.1128/JB.183.6.1835-1842.2001. View

Jeon Y, Lee Y, Han J, Kim J, Hwang D . Multimerization of phosphorylated and non-phosphorylated ArcA is necessary for the response regulator function of the Arc two-component signal transduction system. J Biol Chem. 2001; 276(44):40873-9. DOI: 10.1074/jbc.M104855200. View

Blanco A, Sola M, Gomis-Ruth F, Coll M . Tandem DNA recognition by PhoB, a two-component signal transduction transcriptional activator. Structure. 2002; 10(5):701-13. DOI: 10.1016/s0969-2126(02)00761-x. View

10.

Chen Y, Birck C, Samama J, Hulett F . Residue R113 is essential for PhoP dimerization and function: a residue buried in the asymmetric PhoP dimer interface determined in the PhoPN three-dimensional crystal structure. J Bacteriol. 2002; 185(1):262-73. PMC: 141829. DOI: 10.1128/JB.185.1.262-273.2003. View

11.

Zhang J, Xiao G, Gunsalus R, Hubbell W . Phosphorylation triggers domain separation in the DNA binding response regulator NarL. Biochemistry. 2003; 42(9):2552-9. DOI: 10.1021/bi0272205. View

12.

Minagawa S, Ogasawara H, Kato A, Yamamoto K, Eguchi Y, Oshima T . Identification and molecular characterization of the Mg2+ stimulon of Escherichia coli. J Bacteriol. 2003; 185(13):3696-702. PMC: 161583. DOI: 10.1128/JB.185.13.3696-3702.2003. View

13.

Lejona S, Aguirre A, Cabeza M, Garcia Vescovi E, Soncini F . Molecular characterization of the Mg2+-responsive PhoP-PhoQ regulon in Salmonella enterica. J Bacteriol. 2003; 185(21):6287-94. PMC: 219391. DOI: 10.1128/JB.185.21.6287-6294.2003. View

14.

Aiba H, Nakasai F, Mizushima S, Mizuno T . Phosphorylation of a bacterial activator protein, OmpR, by a protein kinase, EnvZ, results in stimulation of its DNA-binding ability. J Biochem. 1989; 106(1):5-7. DOI: 10.1093/oxfordjournals.jbchem.a122817. View

15.

Makino K, Shinagawa H, Amemura M, Kawamoto T, Yamada M, Nakata A . Signal transduction in the phosphate regulon of Escherichia coli involves phosphotransfer between PhoR and PhoB proteins. J Mol Biol. 1989; 210(3):551-9. DOI: 10.1016/0022-2836(89)90131-9. View

16.

Ireton K, Rudner D, Siranosian K, Grossman A . Integration of multiple developmental signals in Bacillus subtilis through the Spo0A transcription factor. Genes Dev. 1993; 7(2):283-94. DOI: 10.1101/gad.7.2.283. View

17.

Da Re S, Bertagnoli S, Fourment J, Reyrat J, Kahn D . Intramolecular signal transduction within the FixJ transcriptional activator: in vitro evidence for the inhibitory effect of the phosphorylatable regulatory domain. Nucleic Acids Res. 1994; 22(9):1555-61. PMC: 308029. DOI: 10.1093/nar/22.9.1555. View

18.

Asayama M, Yamamoto A, Kobayashi Y . Dimer form of phosphorylated Spo0A, a transcriptional regulator, stimulates the spo0F transcription at the initiation of sporulation in Bacillus subtilis. J Mol Biol. 1995; 250(1):11-23. DOI: 10.1006/jmbi.1995.0354. View

19.

Soncini F, Vescovi E, Groisman E . Transcriptional autoregulation of the Salmonella typhimurium phoPQ operon. J Bacteriol. 1995; 177(15):4364-71. PMC: 177185. DOI: 10.1128/jb.177.15.4364-4371.1995. View

20.

Fiedler U, Weiss V . A common switch in activation of the response regulators NtrC and PhoB: phosphorylation induces dimerization of the receiver modules. EMBO J. 1995; 14(15):3696-705. PMC: 394444. DOI: 10.1002/j.1460-2075.1995.tb00039.x. View