A Method of Enhancing Verocytotoxin Production by Escherichia Coli

Overview

Journal FEMS Microbiol Lett

Publisher Oxford University Press

Specialty Microbiology

Date 1992 Jun 1

PMID 1505736

Citations 13

Authors

D A Burke

S M Scotland

H Al-Mardini

C O Record

Affiliations

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Abstract

The number of verocytotoxin producing Escherichia coli (VTEC) present in the faeces during an infection may be very low, making their detection difficult. We report a method for enhancing toxin production by VTEC using mitomycin C as an inducing agent with the aim of improving the detection of VTEC. In pure culture, mitomycin C enhanced toxin production up to 100-fold. When applied to mixed faecal culture, toxin could be detected in mitomycin C treated samples when standard cultures were negative and when substantially fewer verocytotoxin-producing bacteria were present. Use of this method may aid in the detection of VTEC and is appropriate for use in the routine diagnostic laboratory.

Citing Articles

Shiga Toxin (Verotoxin)-producing   and Foodborne Disease:  A Review.

Terajima J, Izumiya H, Hara-Kudo Y, Ohnishi M Food Saf (Tokyo). 2020; 5(2):35-53.

PMID: 32231928 PMC: 6989183. DOI: 10.14252/foodsafetyfscj.2016029.

The EspF N-Terminal of Enterohemorrhagic O157:H7 EDL933w Imparts Stronger Toxicity Effects on HT-29 Cells than the C-Terminal.

Wang X, Du Y, Hua Y, Fu M, Niu C, Zhang B Front Cell Infect Microbiol. 2017; 7:410.

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The N-terminal domain of EspF induces host cell apoptosis after infection with enterohaemorrhagic Escherichia coli O157:H7.

Zhao S, Zhou Y, Wang C, Yang Y, Wu X, Wei Y PLoS One. 2013; 8(1):e55164.

PMID: 23372831 PMC: 3555930. DOI: 10.1371/journal.pone.0055164.

Shiga toxins and the pathophysiology of hemolytic uremic syndrome in humans and animals.

Mayer C, Leibowitz C, Kurosawa S, Stearns-Kurosawa D Toxins (Basel). 2012; 4(11):1261-87.

PMID: 23202315 PMC: 3509707. DOI: 10.3390/toxins4111261.

Shiga toxin-producing Escherichia coli-associated kidney failure in a 40-year-old patient and late diagnosis by novel bacteriologic and toxin detection methods.

Teel L, Steinberg B, Aronson N, OBrien A J Clin Microbiol. 2003; 41(7):3438-40.

PMID: 12843115 PMC: 165378. DOI: 10.1128/JCM.41.7.3438-3440.2003.