A Reciprocal Translocation, Induced by a Canonical Integration of a Single T-DNA, Interrupts the HMG-I/Y Arabidopsis Thaliana Gene

Major chromosomal rearrangements occur during Arabidopsis thaliana T-DNA transformation. They generally result from interactions between multiple T-DNA copies during the integration process or from aborted integration events. We report here a reciprocal translocation associated with the integration of a single T-DNA which otherwise shows all the characteristic features of a canonical integration event. The exchanged fragments roughly correspond to half of the left arm of chromosome 1 and to two thirds of the right arm of chromosome 2. The chromosome 1 breakpoint maps close to position 23.6 cM and interrupts the coding sequence of the HMG-I/Y gene, which is present at a single copy in the Arabidopsis genome and encodes a non-histone chromosomal protein putatively involved in regulation of gene expression. The chromosome 2 breakpoint maps close to position 33.6 cM, and is located 419 bp upstream of a gene encoding a putative homeodomain transcription factor. Homozygotes for the translocation display a severe phenotype with major developmental abnormalities and total sterility, while heterozygotes are fertile, most of them showing a wild-type phenotype. Among the six possible unbalanced genotypic classes, four are entirely lethal while only a few individuals from the two others survive. Analysis of relations between phenotypes and genotypes strongly suggests that the major phenotypic alterations observed do not result from inactivation of the HMG-I/Y gene.