Similarities and Differences Between the Subgenomic and Minus-strand Promoters of an RNA Plant Virus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2004 Mar 30

PMID 15047821

Citations 9

Authors

Rene C L Olsthoorn

P C Joost Haasnoot

John F Bol

Affiliations

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Abstract

Promoter regions required for minus-strand and subgenomic RNA synthesis have been mapped for several plus-strand RNA viruses. In general, the two types of promoters do not share structural features even though they are recognized by the same viral polymerase. The minus-strand promoter of Alfalfa mosaic virus (AMV), a plant virus of the family Bromoviridae, consists of a triloop hairpin (hpE) which is attached to a 3' tRNA-like structure (TLS). In contrast, the AMV subgenomic promoter consists of a single triloop hairpin that bears no sequence homology with hpE. Here we show that hpE, when detached from its TLS, can function as a subgenomic promoter in vitro and can replace the authentic subgenomic promoter in the live virus. Thus, the AMV subgenomic and minus-strand promoters are basically the same, but the minus-strand promoter is linked to a 3' TLS to force the polymerase to initiate at the very 3'end.

Citing Articles

Evolutionary history and global spatiotemporal pattern of alfalfa mosaic virus.

Gao Y, Fan G, Cheng S, Zhang W, Bai Y Front Microbiol. 2023; 13:1051834.

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Impact of the Potential mA Modification Sites at the 3'UTR of Alfalfa Mosaic Virus RNA3 in the Viral Infection.

Alvarado-Marchena L, Martinez-Perez M, Ubeda J, Pallas V, Aparicio F Viruses. 2022; 14(8).

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An expanded class of histidine-accepting viral tRNA-like structures.

Langeberg C, Sherlock M, Macfadden A, Kieft J RNA. 2021; 27(6):653-664.

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Prediction of putative regulatory elements in the subgenomic promoters of cucumber green mottle mosaic virus and their interactions with the RNA dependent RNA polymerase domain.

Chattopadhyay A, Kader Jailani A, Roy A, Mukherjee S, Mandal B Virusdisease. 2020; 31(4):503-516.

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The subgenomic promoter of brome mosaic virus folds into a stem-loop structure capped by a pseudo-triloop that is structurally similar to the triloop of the genomic promoter.

Skov J, Gaudin M, Podbevsek P, Olsthoorn R, Petersen M RNA. 2012; 18(5):992-1000.

PMID: 22393035 PMC: 3334706. DOI: 10.1261/rna.029918.111.

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