Cestrum Yellow Leaf Curling Virus (CmYLCV) Promoter: a New Strong Constitutive Promoter for Heterologous Gene Expression in a Wide Variety of Crops

Overview

Journal Plant Mol Biol

Date 2004 Mar 11

PMID 15010605

Citations 34

Authors

Livia Stavolone

Maria Kononova

Sandra Pauli

Antonio Ragozzino

Peter de Haan

Steve Milligan

Kay Lawton

Thomas Hohn

Affiliations

Soon will be listed here.

Abstract

Appropriately regulated gene expression requires a suitable promoter. A number of promoters have been isolated and shown to be functional in plants, but only a few of them activate transcription of transgenes at high levels constitutively. We report here the cloning and characterization of a novel, constitutively expressed promoter isolated from Cestrum yellow leaf curling virus (CmYLCV), a double-stranded DNA plant pararetrovirus belonging to the Caulimoviridae family. The CmYLCV promoter is highly active in callus, meristems and vegetative and reproductive tissues in Arabidopsis thaliana, Nicotiana tabacum, Lycopersicon esculentum, Zea mays and Oryza sativa. Furthermore, the level of expression is comparable to, or higher than, that from the CaMV 35S, the 'super-promoter' or the maize ubiquitin 1 promoters, three frequently used promoters in agricultural biotechnology. The heritable, strong and constitutive activity in both monocotyledonous and dicotyledonous plants, combined with the extremely narrow CmYLCV host range, makes the CmYLCV promoter an attractive tool for regulating transgene expression in a wide variety of plant species.

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