Magnetosome Vesicles Are Present Before Magnetite Formation, and MamA is Required for Their Activation

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2004 Mar 9

PMID 15004275

Citations 104

Authors

Arash Komeili

Hojatollah Vali

Terrance J Beveridge

Dianne K Newman

Affiliations

Soon will be listed here.

Abstract

Bacterial magnetosomes are intracellular compartments that house highly ordered magnetite crystals. By using Magnetospirillum sp. AMB-1 as a model system, we show that magnetosome vesicles exist in the absence of magnetite, biomineralization of magnetite proceeds simultaneously in multiple vesicles, and biomineralization proceeds from the same location in each vesicle. The magnetosome-associated protein, MamA, is required for the formation of functional magnetosome vesicles and displays a dynamic subcellular localization throughout the growth cycle of magnetotactic bacteria. Together, these results suggest that the magnetosome precisely coordinates magnetite biomineralization and can serve as a model system for the study of organelle biogenesis in noneukaryotic cells.

Citing Articles

A network of coiled-coil and actin-like proteins controls the cellular organization of magnetosome organelles in deep-branching magnetotactic bacteria.

Russell V, Iavarone A, Ozyamak E, Grant C, Komeili A bioRxiv. 2025; .

PMID: 40060654 PMC: 11888303. DOI: 10.1101/2025.02.24.639979.

Magnetochrome-catalyzed oxidation of ferrous iron by MamP enables magnetite crystal growth in the magnetotactic bacterium AMB-1.

Amor M, Chevrier D, Siponen M, Egli R, Scoppola E, Marcano L Proc Natl Acad Sci U S A. 2024; 121(50):e2410245121.

PMID: 39621904 PMC: 11648623. DOI: 10.1073/pnas.2410245121.

Intrinsic and extrinsic determinants of conditional localization of Mms6 to magnetosome organelles in AMB-1.

Bickley C, Wan J, Komeili A J Bacteriol. 2024; 206(6):e0000824.

PMID: 38819153 PMC: 11332177. DOI: 10.1128/jb.00008-24.

Ferromagnetic resonance spectra of linear magnetosome chains.

Gubanova E, Usov N Beilstein J Nanotechnol. 2024; 15:157-167.

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Live-Cell Fluorescence Imaging of Magnetosome Organelle for Magnetotaxis Motility.

Eguchi Y, Taoka A Methods Mol Biol. 2023; 2646:133-146.

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