Multiplex PCR Assay for Direct Identification of Group B Streptococcal Alpha-protein-like Protein Genes

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2004 Mar 9

PMID 15004110

Citations 65

Authors

Roberta Creti

Francesca Fabretti

Graziella Orefici

Christina Von Hunolstein

Affiliations

Soon will be listed here.

Abstract

We developed a group B streptococcus multiplex PCR assay which allows, by direct analysis of the amplicon size, determination of the surface protein antigen genes of alpha-C protein, epsilon protein, Rib, Alp2, Alp3, and Alp4. The multiplex PCR assay offers a rapid and simple method of subtyping Streptococcus agalactiae based on surface protein genes.

Citing Articles

Unveiling the genetic landscape of Streptococcus agalactiae bacteremia: emergence of hypervirulent CC1 strains and new CC283 strains in Tehran, Iran.

Mohammadi A, Amini C, Bagheri P, Salehi Z, Goudarzi M BMC Microbiol. 2024; 24(1):365.

PMID: 39342084 PMC: 11438095. DOI: 10.1186/s12866-024-03521-z.

Group B Streptococcus Infections in Non-Pregnant Adults, Italy, 2015-2019.

Imperi M, Gherardi G, Alfarone G, Creti R Pathogens. 2024; 13(9).

PMID: 39338998 PMC: 11434888. DOI: 10.3390/pathogens13090807.

Emergence of High-Level Gentamicin Resistance in Hypervirulent Serotype IV ST1010 (CC452) Strains by Acquisition of a Novel Integrative and Conjugative Element.

Creti R, Imperi M, Basit Khan U, Berardi A, Recchia S, Alfarone G Antibiotics (Basel). 2024; 13(6).

PMID: 38927158 PMC: 11201010. DOI: 10.3390/antibiotics13060491.

Incidence of invasive infections with Group B streptococcus in adults in Norway 1996-2019: a nationwide registry-based case-control study.

Uggen E, Olaisen C, Lyng R, Simonsen G, Baevre-Jensen R, Gran F Infection. 2024; 52(5):1745-1752.

PMID: 38483786 PMC: 11499455. DOI: 10.1007/s15010-024-02210-3.

Macrolide and lincosamide resistance of Streptococcus agalactiae in pregnant women in Poland.

Kaminska D, Ratajczak M, Nowak-Malczewska D, Karolak J, Kwasniewski M, Szumala-Kakol A Sci Rep. 2024; 14(1):3877.

PMID: 38366099 PMC: 10873391. DOI: 10.1038/s41598-024-54521-y.

References

Kong F, Gowan S, Martin D, James G, Gilbert G . Serotype identification of group B streptococci by PCR and sequencing. J Clin Microbiol. 2002; 40(1):216-26. PMC: 120111. DOI: 10.1128/JCM.40.1.216-226.2002. View

Gravekamp C, Kasper D, Paoletti L, Madoff L . Alpha C protein as a carrier for type III capsular polysaccharide and as a protective protein in group B streptococcal vaccines. Infect Immun. 1999; 67(5):2491-6. PMC: 115995. DOI: 10.1128/IAI.67.5.2491-2496.1999. View

Bolduc G, Baron M, Gravekamp C, Lachenauer C, Madoff L . The alpha C protein mediates internalization of group B Streptococcus within human cervical epithelial cells. Cell Microbiol. 2002; 4(11):751-8. DOI: 10.1046/j.1462-5822.2002.00227.x. View

Baker C, Edwards M . Group B streptococcal conjugate vaccines. Arch Dis Child. 2003; 88(5):375-8. PMC: 1719562. DOI: 10.1136/adc.88.5.375. View

Paoletti L, Kasper D . Glycoconjugate vaccines to prevent group B streptococcal infections. Expert Opin Biol Ther. 2003; 3(6):975-84. DOI: 10.1517/14712598.3.6.975. View

Ferrieri P . Surface-localized protein antigens of group B streptococci. Rev Infect Dis. 1988; 10 Suppl 2:S363-6. DOI: 10.1093/cid/10.supplement_2.s363. View

Heden L, Frithz E, Lindahl G . Molecular characterization of an IgA receptor from group B streptococci: sequence of the gene, identification of a proline-rich region with unique structure and isolation of N-terminal fragments with IgA-binding capacity. Eur J Immunol. 1991; 21(6):1481-90. DOI: 10.1002/eji.1830210623. View

Jerlstrom P, Chhatwal G, Timmis K . The IgA-binding beta antigen of the c protein complex of Group B streptococci: sequence determination of its gene and detection of two binding regions. Mol Microbiol. 1991; 5(4):843-9. DOI: 10.1111/j.1365-2958.1991.tb00757.x. View

Michel J, Madoff L, Olson K, Kling D, Kasper D, Ausubel F . Large, identical, tandem repeating units in the C protein alpha antigen gene, bca, of group B streptococci. Proc Natl Acad Sci U S A. 1992; 89(21):10060-4. PMC: 50277. DOI: 10.1073/pnas.89.21.10060. View

10.

Madoff L, Michel J, Gong E, Kling D, Kasper D . Group B streptococci escape host immunity by deletion of tandem repeat elements of the alpha C protein. Proc Natl Acad Sci U S A. 1996; 93(9):4131-6. PMC: 39499. DOI: 10.1073/pnas.93.9.4131. View

11.

Wastfelt M, Stalhammar-Carlemalm M, Delisse A, Cabezon T, Lindahl G . Identification of a family of streptococcal surface proteins with extremely repetitive structure. J Biol Chem. 1996; 271(31):18892-7. DOI: 10.1074/jbc.271.31.18892. View

12.

Larsson C, Stalhammar-Carlemalm M, Lindahl G . Experimental vaccination against group B streptococcus, an encapsulated bacterium, with highly purified preparations of cell surface proteins Rib and alpha. Infect Immun. 1996; 64(9):3518-23. PMC: 174257. DOI: 10.1128/iai.64.9.3518-3523.1996. View

13.

Lachenauer C, Creti R, Michel J, Madoff L . Mosaicism in the alpha-like protein genes of group B streptococci. Proc Natl Acad Sci U S A. 2000; 97(17):9630-5. PMC: 16916. DOI: 10.1073/pnas.97.17.9630. View

14.

Spellerberg B . Pathogenesis of neonatal Streptococcus agalactiae infections. Microbes Infect. 2001; 2(14):1733-42. DOI: 10.1016/s1286-4579(00)01328-9. View

15.

Lachenauer C, Madoff L . A protective surface protein from type V group B streptococci shares N-terminal sequence homology with the alpha C protein. Infect Immun. 1996; 64(10):4255-60. PMC: 174365. DOI: 10.1128/iai.64.10.4255-4260.1996. View

16.

Schuchat A . Group B streptococcus. Lancet. 1999; 353(9146):51-6. DOI: 10.1016/S0140-6736(98)07128-1. View

17.

Kong F, Gowan S, Martin D, James G, Gilbert G . Molecular profiles of group B streptococcal surface protein antigen genes: relationship to molecular serotypes. J Clin Microbiol. 2002; 40(2):620-6. PMC: 153396. DOI: 10.1128/JCM.40.2.620-626.2002. View