Ontogeny of Glucagon Messenger RNA in the Rat Pancreas

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1992 Jan 1

PMID 1500299

Authors

S L Jin

M A Hynes

J G Simmons

J M Lauder

P K Lund

Affiliations

Soon will be listed here.

Abstract

The synthesis of proglucagon mRNA was studied in rat pancreas from day 11 of fetal gestation (E11) to maturity. Proglucagon mRNA was first detected on E11, the time that the pancreatic bud forms in developing rats. The synthesis of proglucagon mRNA and its translation product at this early time point in pancreatic development suggests an early differentiation of A cell function. Between E17 prenatally and day 10-14 postnatally, pancreatic proglucagon mRNA abundance was higher than in adult pancreas. Regulation of the abundance of pancreatic proglucagon mRNA therefore appears to underlie the previously documented increases in serum and pancreatic glucagon immunoreactivity in the late fetal and perinatal periods. By day 20 postnatally, pancreatic proglucagon mRNA declined to levels found in adult pancreas. Prenatally between E17 and E21, changes in proglucagon mRNA abundance did not parallel previously reported developmental changes in relative mass of proglucagon-producing pancreatic A cells. This suggests that changes in proglucagon mRNA abundance during these times may be attributed to changes in proglucagon gene transcription or proglucagon mRNA stability per cell. In contrast between E21 and maturity, changes in proglucagon mRNA abundance paralleled previously reported changes in relative A cell mass, suggesting no major changes in proglucagon gene transcription or mRNA stability per cell during these times.

References

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Yoshinari M, Daikoku S . Ontogenetic appearance of immunoreactive endocrine cells in rat pancreatic islets. Anat Embryol (Berl). 1982; 165(1):63-70. DOI: 10.1007/BF00304583. View

Lund P, Simmons J, Hoyt E, DErcole A, Martin F, Van Wyk J . Nucleotide sequence analysis of a cDNA encoding human ubiquitin reveals that ubiquitin is synthesized as a precursor. J Biol Chem. 1985; 260(12):7609-13. View

SCHWEISTHAL M, Frost C, Brinn J . Ontogeny of four cell types in fetal rat islets using histochemical techniques. Acta Diabetol Lat. 1976; 13(1-2):30-9. DOI: 10.1007/BF02591579. View

Rethelyi M, METZ C, Lund P . Distribution of neurons expressing calcitonin gene-related peptide mRNAs in the brain stem, spinal cord and dorsal root ganglia of rat and guinea-pig. Neuroscience. 1989; 29(1):225-39. DOI: 10.1016/0306-4522(89)90345-x. View

Thomas P . Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980; 77(9):5201-5. PMC: 350025. DOI: 10.1073/pnas.77.9.5201. View

Aviv H, Leder P . Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972; 69(6):1408-12. PMC: 426713. DOI: 10.1073/pnas.69.6.1408. View

Heinrich G, Gros P, Lund P, Bentley R, Habener J . Pre-proglucagon messenger ribonucleic acid: nucleotide and encoded amino acid sequences of the rat pancreatic complementary deoxyribonucleic acid. Endocrinology. 1984; 115(6):2176-81. DOI: 10.1210/endo-115-6-2176. View

Fujii S . Development of pancreatic endocrine cells in the rat fetus. Arch Histol Jpn. 1979; 42(4):467-79. DOI: 10.1679/aohc1950.42.467. View

10.

Unger R, Orci L . Glucagon and the A cell: physiology and pathophysiology (first two parts). N Engl J Med. 1981; 304(25):1518-24. DOI: 10.1056/NEJM198106183042504. View

11.

McEvoy R, Madson K . Pancreatic insulin-, glucagon-, and somatostatin-positive islet cell populations during the perinatal development of the rat. II. Changes in hormone content and concentration. Biol Neonate. 1980; 38(5-6):255-9. DOI: 10.1159/000241373. View

12.

McEvoy R . Changes in the volumes of the A-, B-, and D-cell populations in the pancreatic islets during the postnatal development of the rat. Diabetes. 1981; 30(10):813-7. DOI: 10.2337/diab.30.10.813. View

13.

Muller W, Faloona G, Unger R . Abnormal alpha-cell function in diabetes. Response to carbohydrate and protein ingestion. N Engl J Med. 1970; 283(3):109-15. DOI: 10.1056/NEJM197007162830301. View

14.

Efrat S, Teitelman G, Anwar M, Ruggiero D, Hanahan D . Glucagon gene regulatory region directs oncoprotein expression to neurons and pancreatic alpha cells. Neuron. 1988; 1(7):605-13. DOI: 10.1016/0896-6273(88)90110-9. View

15.

Chan S, Noyes B, Agarwal K, Steiner D . Construction and selection of recombinant plasmids containing full-length complementary DNAs corresponding to rat insulins I and II. Proc Natl Acad Sci U S A. 1979; 76(10):5036-40. PMC: 413074. DOI: 10.1073/pnas.76.10.5036. View

16.

Lund P, Hynes M, Simmons J, Jansen M, DErcole A, Van Wyk J . Somatomedin-C/insulin-like growth factor-I and insulin-like growth factor-II mRNAs in rat fetal and adult tissues. J Biol Chem. 1986; 261(31):14539-44. View

17.

Crabtree G, Kant J . Organization of the rat gamma-fibrinogen gene: alternative mRNA splice patterns produce the gamma A and gamma B (gamma ') chains of fibrinogen. Cell. 1982; 31(1):159-66. DOI: 10.1016/0092-8674(82)90415-9. View

18.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

19.

Ullrich A, Shine J, Chirgwin J, Pictet R, Tischer E, Rutter W . Rat insulin genes: construction of plasmids containing the coding sequences. Science. 1977; 196(4296):1313-9. DOI: 10.1126/science.325648. View

20.

McEvoy R, Madson K . Pancreatic insulikn-, glucagon-, and somatostatin-positive islet cell populatins during the perinatal development of the rat. I. Morphometric quantitation. Biol Neonate. 1980; 38(5-6):248-54. DOI: 10.1159/000241372. View