Nitric Oxide-mediated Modification of the Glycine Binding Site of the NMDA Receptor During Hypoxia in the Cerebral Cortex of the Newborn Piglet

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 2004 Mar 9

PMID 15002744

Citations 1

Authors

David F Sorrentino

Karen I Fritz

Syed Hassan Haider

Nehal Parikh

Maria Delivoria Papadopoulos

Om P Mishra

Affiliations

Soon will be listed here.

Abstract

This study tested the hypothesis that cerebral hypoxia results in nitric oxide (NO)-mediated modification of the glycine-binding site of the N-methyl-D-aspartate (NMDA) receptor. Glycine binding characteristics were determined in normoxic, hypoxic, and hypoxic with 7-nitroindazole (7-NINA)-pretreated newborn piglets. The role of nitration was evaluated by determining binding characteristics in non-nitrated and in-vitro nitrated membranes. Bmax and Kd values were 30% higher in the hypoxic group than the normoxic and 7-NINA pretreated hypoxic groups. Kd values in the in-vitro normoxic nitrated membranes were similar to the non-nitrated hypoxic group. Bmax values in the in-vitro) normoxic nitrated membrane samples were 16% lower than in the non-nitrated hypoxic group. We conclude cerebral hypoxia causes modification of the glycine-binding site of the NMDA receptor and this modification of the glycine-binding site may be NO mediated. We propose that NO-mediated modification of the glycine-binding site of the NMDA receptor regulates calcium influx through its ion-channel.

Citing Articles

Effect of 7-nitroindazole sodium on the cellular distribution of neuronal nitric oxide synthase in the cerebral cortex of hypoxic newborn piglets.

Katsetos C, Parikh N, Fritz K, Legido A, Delivoria-Papadopoulos M, Mishra O Neurochem Res. 2006; 31(7):899-906.

PMID: 16804757 DOI: 10.1007/s11064-006-9094-y.

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