Nuclear Suppressors of the [poky] Cytoplasmic Mutant in Neurospora Crassa. III. Effects on Other Cytoplasmic Mutants and on Mitochondrial Ribosome Assembly in [poky]

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1978 May 31

PMID 149910

Citations 11

Authors

R A Collins

H Bertrand

Affiliations

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Abstract

We have previously isolated six non-allelic, nuclear mutations (sui loci) that partially suppress the growth, respiratory and cytochrome abnormalities of the extranuclear [poky] mutant. A comparison of the mitochondrial ribosome profiles of suppressed and unsuppressed [poky] strains revealed that five of the six suppressors alleviate at least partially the deficiency of mitochondrial small ribosomal subunits that is associated with the [poky] genotype. Six independently isolated Group 1 extranuclear mutants, namely [exn-1], [exn-2], [exn-4-a1, [stp-b1], [SG-1] and [SG-3-A1, which have growth and cytochrome phenotypes similar to [poky] also were found to be deficient in small subunits of mitochondrial ribosomes. Using cytochrome aa3 and b production as a criterion for mitochondrial protein synthesis, it could be shown that the nuclear su I suppressors of [poky] also suppress the other six Group I extranuclear mutants. However, differences in the efficiencies of suppression by suI suppressors suggest that at least some of Group I extrachromosomal mutants are not simply re-isolates of [poky], but represent distinct extranuclear mutations.

Citing Articles

Temperature-sensitive nuclear mutants of Neurospora crassa deficient in mitochondrial ribosomes.

Nargang F, Bertrand H, Collins R Curr Genet. 2013; 1(1):1-7.

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Genetic interactions in the control of mitochondrial function in Paramecium. II. Interactions between nuclear and mitochondrial genomes.

Ruiz F, Knowles J Mol Gen Genet. 1980; 180(3):563-72.

PMID: 6936602 DOI: 10.1007/BF00268061.

A novel extranuclear mutant of Neurospora with a temperature-sensitive defect in mitochondrial protein synthesis and mitochondrial ATPase.

Collins R, Bertrand H, LaPolla R, Lambowitz A Mol Gen Genet. 1981; 181(1):13-9.

PMID: 6261083 DOI: 10.1007/BF00338998.

The [poky] mutant of Neurospora contains a 4-base-pair deletion at the 5' end of the mitochondrial small rRNA.

Akins R, Lambowitz A Proc Natl Acad Sci U S A. 1984; 81(12):3791-5.

PMID: 6233613 PMC: 345306. DOI: 10.1073/pnas.81.12.3791.

Chromosomal loci of Neurospora crassa.

Perkins D, Radford A, Newmeyer D, Bjorkman M Microbiol Rev. 1982; 46(4):426-570.

PMID: 6219280 PMC: 281555. DOI: 10.1128/mr.46.4.426-570.1982.

References

Kohout J, Bertrand H . Nuclear suppressors of the [poky] cytoplasmic mutant in Neurospora crassa. I. Genetics and respiratory properties. Can J Genet Cytol. 1976; 18(2):311-24. DOI: 10.1139/g76-039. View

Mitchell M, MITCHELL H . A nuclear gene suppressor of a cytoplasmically inherited character in Neurospora crassa. J Gen Microbiol. 1956; 14(1):84-9. DOI: 10.1099/00221287-14-1-84. View

Bertrand H, Kohout J . Nuclear suppressors of the (poky) cytoplasmic mutant in Neurospora crassa. II. Mitochondrial cytochrome systems. Can J Genet Cytol. 1977; 19(1):81-91. DOI: 10.1139/g77-010. View

Kuriyama Y, Luck D . Ribosomal RNA synthesis in mitochondria of Neurospora crassa. J Mol Biol. 1973; 73(4):425-37. DOI: 10.1016/0022-2836(73)90091-0. View

SCHAFER K, Kuntzel H . Mitochondrial genes in neurospora: a single cistron for ribosomal RNA. Biochem Biophys Res Commun. 1972; 46(3):1312-9. DOI: 10.1016/s0006-291x(72)80118-9. View

Lambowitz A, Chua N, Luck D . Mitochondrial ribosome assembly in Neurospora. Preparation of mitochondrial ribosomal precursor particles, site of synthesis of mitochondrial ribosomal proteins and studies on the poky mutant. J Mol Biol. 1976; 107(3):223-53. DOI: 10.1016/s0022-2836(76)80003-4. View

Bertrand H, Pittenger T . Cytoplasmic Mutants Selected from Continuously Growing Cultures of NEUROSPORA CRASSA. Genetics. 1969; 61(3):643-59. PMC: 1212231. DOI: 10.1093/genetics/61.3.643. View

Sebald W, Bucher T, Olbrich B, Kaudewitz F . Electrophoretic pattern of and amino acid incorporation in vitro into the insoluble mitochondrial protein of neurospora crassa wild type and mi-1 mutant. FEBS Lett. 1968; 1(4):235-240. DOI: 10.1016/0014-5793(68)80071-7. View

Davies J, Nomura M . The genetics of bacterial ribosomes. Annu Rev Genet. 1972; 6:203-34. DOI: 10.1146/annurev.ge.06.120172.001223. View

10.

Saltzman L, Brown M, Apirion D . Functional interdependence among ribosomal elements as revealed by genetic analysis. Mol Gen Genet. 1974; 133(3):201-7. DOI: 10.1007/BF00267669. View

11.

Weiss H, Ziganke B . Cytochrome b in Neurospora crassa mitochondria. Site of translation of the heme protein. Eur J Biochem. 1974; 41(1):63-71. DOI: 10.1111/j.1432-1033.1974.tb03244.x. View

12.

Gornall A, BARDAWILL C, DAVID M . Determination of serum proteins by means of the biuret reaction. J Biol Chem. 1949; 177(2):751-66. View

13.

Bertrand H, Pittenger T . Isolation and classification of extranuclear mutants of Neurospora crassa. Genetics. 1972; 71(4):521-33. PMC: 1212791. DOI: 10.1093/genetics/71.4.521. View

14.

Lizardi P, Luck D . The intracellular site of synthesis of mitochndrial ribosomal proteins in Neurospora crassa. J Cell Biol. 1972; 54(1):56-74. PMC: 2108856. DOI: 10.1083/jcb.54.1.56. View

15.

Isono K . Altered 30S ribosomal proteins in streptomycin resistant, dependent and independent mutants of Bacillus stearothermophilus. Mol Gen Genet. 1974; 133(1):77-86. DOI: 10.1007/BF00268679. View

16.

Rifkin M, Luck D . Defective production of mitochondrial ribosomes in the poky mutant of Neurospora crassa. Proc Natl Acad Sci U S A. 1971; 68(2):287-90. PMC: 388919. DOI: 10.1073/pnas.68.2.287. View

17.

Bertrand H, Pittenger T . Complementation among cytoplasmic mutants of Neurospora crassa. Mol Gen Genet. 1972; 117(1):82-90. DOI: 10.1007/BF00268840. View

18.

Mitchell M, MITCHELL H . A Case of "Maternal" Inheritance in Neurospora Crassa. Proc Natl Acad Sci U S A. 1952; 38(5):442-9. PMC: 1063583. DOI: 10.1073/pnas.38.5.442. View

19.

Sebald W, Machleidt W, Otto J . Products of mitochondrial protein synthesis in Neurospora crassa. Determination of equimolar amounts of three products in cytochrome oxidase on the basis of amino-acid analysis. Eur J Biochem. 1973; 38(2):311-24. DOI: 10.1111/j.1432-1033.1973.tb03064.x. View

20.

Lambowitz A, SLAYMAN C . Cyanide-resistant respiration in Neurospora crassa. J Bacteriol. 1971; 108(3):1087-96. PMC: 247191. DOI: 10.1128/jb.108.3.1087-1096.1971. View