The Arabidopsis Cytosolic Thioredoxin H5 Gene Induction by Oxidative Stress and Its W-box-mediated Response to Pathogen Elicitor

Overview

Journal Plant Physiol

Specialty Physiology

Date 2004 Feb 21

PMID 14976236

Citations 86

Authors

Christophe Laloi

Dominique Mestres-Ortega

Yves Marco

Yves Meyer

Jean-Philippe Reichheld

Affiliations

Soon will be listed here.

Abstract

The AtTRXh5 protein belongs to the cytosolic thioredoxins h family that, in Arabidopsis, contains eight members showing very distinct patterns and levels of expression. Here, we show that the AtTRXh5 gene is up-regulated during wounding, abscission, and senescence, as well as during incompatible interactions with the bacterial pathogen Pseudomonas syringae. By electrophoretic mobility shift assays, a binding activity on a W-box in the AtTRXh5 promoter region was found induced by treatments with the P. syringae-derived elicitor peptide flg22, suggesting that a WRKY transcription factor controls AtTRXh5 induction upon elicitor treatment. Remarkably, AtTRXh5 was up-regulated in plants overexpressing WRKY6. More generally, AtTRXh5 is induced in response to oxidative stress conditions. Collectively, our data indicate a possible implication of the cytosolic thioredoxin AtTRXh5 in response to pathogens and to oxidative stresses. In addition, this regulation is unique to AtTRXh5 among the thioredoxin h family, arguing in favor of a speciation rather than to a redundancy of the members of this multigenic family.

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References

Dietz K . Plant peroxiredoxins. Annu Rev Plant Biol. 2003; 54:93-107. DOI: 10.1146/annurev.arplant.54.031902.134934. View

Schutzendubel A, Polle A . Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization. J Exp Bot. 2002; 53(372):1351-65. View

Jiang M, Zhang J . Involvement of plasma-membrane NADPH oxidase in abscisic acid- and water stress-induced antioxidant defense in leaves of maize seedlings. Planta. 2002; 215(6):1022-30. DOI: 10.1007/s00425-002-0829-y. View

Guan L, Scandalios J . Two structurally similar maize cytosolic superoxide dismutase genes, Sod4 and Sod4A, respond differentially to abscisic acid and high osmoticum. Plant Physiol. 1998; 117(1):217-24. PMC: 35006. DOI: 10.1104/pp.117.1.217. View

Broin M, Rey P . Potato plants lacking the CDSP32 plastidic thioredoxin exhibit overoxidation of the BAS1 2-cysteine peroxiredoxin and increased lipid Peroxidation in thylakoids under photooxidative stress. Plant Physiol. 2003; 132(3):1335-43. PMC: 167073. DOI: 10.1104/pp.103.021626. View

Garreton V, Carpinelli J, Jordana X, Holuigue L . The as-1 promoter element is an oxidative stress-responsive element and salicylic acid activates it via oxidative species. Plant Physiol. 2002; 130(3):1516-26. PMC: 166670. DOI: 10.1104/pp.009886. View

Lemaire , Keryer , STEIN , Schepens I , JACQUOT . Heavy-metal regulation of thioredoxin gene expression in chlamydomonas reinhardtii . Plant Physiol. 1999; 120(3):773-8. PMC: 59315. DOI: 10.1104/pp.120.3.773. View

Brugidou C, Marty I, Chartier Y, Meyer Y . The Nicotiana tabacum genome encodes two cytoplasmic thioredoxin genes which are differently expressed. Mol Gen Genet. 1993; 238(1-2):285-93. DOI: 10.1007/BF00279557. View

Ruelland . Regulation of chloroplast enzyme activities by thioredoxins: activation or relief from inhibition?. Trends Plant Sci. 1999; 4(4):136-141. DOI: 10.1016/s1360-1385(99)01391-6. View

10.

Laurent T, Moore E, REICHARD P . ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEOTIDES. IV. ISOLATION AND CHARACTERIZATION OF THIOREDOXIN, THE HYDROGEN DONOR FROM ESCHERICHIA COLI B. J Biol Chem. 1964; 239:3436-44. View

11.

Marx C, Wong J, Buchanan B . Thioredoxin and germinating barley: targets and protein redox changes. Planta. 2003; 216(3):454-60. DOI: 10.1007/s00425-002-0857-7. View

12.

Robatzek S, Somssich I . A new member of the Arabidopsis WRKY transcription factor family, AtWRKY6, is associated with both senescence- and defence-related processes. Plant J. 2001; 28(2):123-33. DOI: 10.1046/j.1365-313x.2001.01131.x. View

13.

Jiang M, Zhang J . Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Plant Cell Physiol. 2001; 42(11):1265-73. DOI: 10.1093/pcp/pce162. View

14.

Bauer Z, Gomez-Gomez L, Boller T, Felix G . Sensitivity of different ecotypes and mutants of Arabidopsis thaliana toward the bacterial elicitor flagellin correlates with the presence of receptor-binding sites. J Biol Chem. 2001; 276(49):45669-76. DOI: 10.1074/jbc.M102390200. View

15.

Kobrehel K, Wong J, Balogh A, Kiss F, Yee B, Buchanan B . Specific reduction of wheat storage proteins by thioredoxin h. Plant Physiol. 1992; 99(3):919-24. PMC: 1080564. DOI: 10.1104/pp.99.3.919. View

16.

Pnueli L, Liang H, Rozenberg M, Mittler R . Growth suppression, altered stomatal responses, and augmented induction of heat shock proteins in cytosolic ascorbate peroxidase (Apx1)-deficient Arabidopsis plants. Plant J. 2003; 34(2):187-203. DOI: 10.1046/j.1365-313x.2003.01715.x. View

17.

Gan S, Amasino R . Inhibition of leaf senescence by autoregulated production of cytokinin. Science. 1995; 270(5244):1986-8. DOI: 10.1126/science.270.5244.1986. View

18.

Konig J, Baier M, Horling F, Kahmann U, Harris G, Schurmann P . The plant-specific function of 2-Cys peroxiredoxin-mediated detoxification of peroxides in the redox-hierarchy of photosynthetic electron flux. Proc Natl Acad Sci U S A. 2002; 99(8):5738-43. PMC: 122841. DOI: 10.1073/pnas.072644999. View

19.

Flohe L, Otting F . Superoxide dismutase assays. Methods Enzymol. 1984; 105:93-104. DOI: 10.1016/s0076-6879(84)05013-8. View

20.

Gomez-Gomez L, Boller T . Flagellin perception: a paradigm for innate immunity. Trends Plant Sci. 2002; 7(6):251-6. DOI: 10.1016/s1360-1385(02)02261-6. View