Characterization of Two Kinases Involved in Thiamine Pyrophosphate and Pyridoxal Phosphate Biosynthesis in Bacillus Subtilis: 4-amino-5-hydroxymethyl-2methylpyrimidine Kinase and Pyridoxal Kinase

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2004 Feb 20

PMID 14973012

Citations 18

Authors

Joo-Heon Park

Kristin Burns

Cynthia Kinsland

Tadhg P Begley

Affiliations

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Abstract

Two Bacillus subtilis genes encoding two proteins (currently annotated ThiD and YjbV) were overexpressed and characterized. YjbV has 4-amino-5-hydroxymethyl-2-methylpyrimidine and 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate kinase activity and should be reannotated ThiD, and B. subtilis ThiD has pyridoxine, pyridoxal, and pyridoxamine kinase activity and should be reannotated PdxK.

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