Ultrastructural Detection in Vitro of WGA-, RCA I-, and Con A-binding Sites Involved in the Invasion of Heart Muscle Cells by Trypanosoma Cruzi

Overview

Journal Parasitol Res

Specialty Parasitology

Date 1992 Jan 1

PMID 1495918

Citations 8

Authors

H S Barbosa

M de N de Meirelles

Affiliations

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Abstract

The presence of carbohydrate residues in the plasma membrane of normal and Trypanosoma cruzi-infected heart muscle cells was investigated cytochemically using ruthenium red, lanthanum nitrate, periodic acid-Schiff/thiocarbohydrazide/silver, and gold- and ferritin-lectin complexes. The study combined conventional electron microscopy with the new analytical technique of electron spectroscopic imaging (ESI). Galactosyl, mannosyl, and sialyl residues were detected in regions of host-cell plasma membrane that undergo interiorization together with the parasite. Lectin-binding sites were sometimes found to show a punctate or patchy distribution in the endocytic vacuole membrane. These findings suggest the that glycoconjugates cytochemically detected in the host-cell plasma membrane participate in the invasion of heart muscle cells by T. cruzi.

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