Nucleolar Organiser Regions As a Marker of Growth Rate in Squamous Cell Carcinoma of the Lung

Overview

Journal Thorax

Date 1992 Oct 1

PMID 1481176

Citations 1

Authors

S Abe

N Sukoh

S Ogura

H Kunikane

N Watanabe

I Nakajima

H Takekawa

Y Kawakami

Affiliations

Soon will be listed here.

Abstract

Background: The numbers of nucleolar organiser regions (AgNORs) per cell has been considered as an indicator of the cellular proliferative activity. A study was carried out to examine whether AgNOR numbers relate to the growth rate in squamous cell carcinoma of the lung.

Methods: AgNORs were stained by a one step silver method, and examined in representative paraffin sections from 45 cases of squamous cell carcinoma of the lung treated by surgical resection of the primary tumour.

Results: The mean (SD) AgNOR numbers per cell in squamous cell carcinomas (5.3 (0.9)) were significantly higher than those in normal bronchial epithelium (1.2 (0.1)). There was no statistical difference among tumours of different post-surgical stages (stage I = 5.2 (0.8), II = 5.9 (1.4), III A = 5.5 (1.3)). The tumour volume doubling time in these cases ranged from 74 to 208 days (120.7 (40.4)). There was a high inverse correlation between the AgNOR numbers and doubling time.

Conclusion: The AgNOR numbers were related to the growth rate of squamous cell carcinoma of the lung. Thus the AgNOR count could be used as a useful marker for investigating the cellular proliferative activity.

Citing Articles

Nucleolar organizer regions in oral squamous cell carcinoma.

Khiavi M, Vosoughhosseini S, Halimi M, Mahmoudi S, Yarahmadi A J Dent Res Dent Clin Dent Prospects. 2012; 6(1):17-20.

PMID: 22991629 PMC: 3442442. DOI: 10.5681/joddd.2012.004.

References

Ogura S, Abe S, Sukoh N, Kunikane H, Nakajima I, Inoue K . Correlation between nucleolar organizer regions visualized by silver staining and the growth rate in lung adenocarcinoma. Cancer. 1992; 70(1):63-8. DOI: 10.1002/1097-0142(19920701)70:1<63::aid-cncr2820700111>3.0.co;2-v. View

Ochs R, Busch H . Further evidence that phosphoprotein C23 (110 kD/pI 5.1) is the nucleolar silver staining protein. Exp Cell Res. 1984; 152(1):260-5. DOI: 10.1016/0014-4827(84)90251-9. View

Ploton D, Menager M, Jeannesson P, Himber G, Pigeon F, Adnet J . Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level. Histochem J. 1986; 18(1):5-14. DOI: 10.1007/BF01676192. View

Leong A, Gilham P . Silver staining of nucleolar organizer regions in malignant melanoma and melanotic nevi. Hum Pathol. 1989; 20(3):257-62. DOI: 10.1016/0046-8177(89)90133-0. View

Derenzini M, Pession A, Farabegoli F, Trere D, Badiali M, Dehan P . Relationship between interphasic nucleolar organizer regions and growth rate in two neuroblastoma cell lines. Am J Pathol. 1989; 134(4):925-32. PMC: 1879788. View

ELLIOTT C, Sloane J, Pallett C, Sanderson K . Cutaneous leucocyte composition after human allogeneic bone marrow transplantation: relationship to marrow purging, histology and clinical rash. Histopathology. 1988; 12(1):1-16. DOI: 10.1111/j.1365-2559.1988.tb01912.x. View

Weiss L, Strickler J, Medeiros L, Gerdes J, STEIN H, Warnke R . Proliferative rates of non-Hodgkin's lymphomas as assessed by Ki-67 antibody. Hum Pathol. 1987; 18(11):1155-9. DOI: 10.1016/s0046-8177(87)80384-2. View

Muggia F, Krezoski S, Hansen H . Cell kinetic studies in patients with small cell carcinoma of the lung. Cancer. 1974; 34(5):1683-90. DOI: 10.1002/1097-0142(197411)34:5<1683::aid-cncr2820340516>3.0.co;2-w. View

Abe S, Isobe H, Ogura S, Nakajima I, Watanabe N, Sukoh N . [Nucleolar organizer regions, nuclear DNA content, and proliferative activity in adenocarcinoma of the lung]. Nihon Kyobu Shikkan Gakkai Zasshi. 1991; 29(10):1282-6. View

10.

Boldy D, Ayres J, Crocker J, Waterhouse J, Gilthorpe M . Interphase nucleolar organiser regions and survival in squamous cell carcinoma of the bronchus: a 10 year follow up study of 138 cases. Thorax. 1991; 46(12):871-7. PMC: 463490. DOI: 10.1136/thx.46.12.871. View

11.

Abe S, Ogura S, Kunikane H, Suko N, Watanabe N, Nakajima I . Nucleolar organizer regions in precancerous and cancerous lesions of the bronchus. Cancer. 1991; 67(2):472-75. DOI: 10.1002/1097-0142(19910115)67:2<472::aid-cncr2820670225>3.0.co;2-n. View

12.

Egan M, Crocker J . Molecular biology and respiratory disease. 3. Evaluation of nucleolar organiser regions in pulmonary pathology. Thorax. 1990; 45(3):225-32. PMC: 462389. DOI: 10.1136/thx.45.3.225. View

13.

Crocker J, Nar P . Nucleolar organizer regions in lymphomas. J Pathol. 1987; 151(2):111-8. DOI: 10.1002/path.1711510203. View

14.

Namikawa R, Ueda R, SUCHI T, Itoh G, Ota K, Takahashi T . Double immunoenzymatic detection of surface phenotype of proliferating lymphocytes in situ with monoclonal antibodies against DNA polymerase alpha and lymphocyte membrane antigens. Am J Clin Pathol. 1987; 87(6):725-31. DOI: 10.1093/ajcp/87.6.725. View

15.

Raymond W, Leong A . Nucleolar organizer regions relate to growth fractions in human breast carcinoma. Hum Pathol. 1989; 20(8):741-6. DOI: 10.1016/0046-8177(89)90066-x. View

16.

Robbins B, de la Vega D, Ogata K, Tan E, Nakamura R . Immunohistochemical detection of proliferating cell nuclear antigen in solid human malignancies. Arch Pathol Lab Med. 1987; 111(9):841-5. View

17.

Crocker J, Macartney J, Smith P . Correlation between DNA flow cytometric and nucleolar organizer region data in non-Hodgkin's lymphomas. J Pathol. 1988; 154(2):151-6. DOI: 10.1002/path.1711540207. View

18.

Sugihara H, Hattori T, Fukuda M . Immunohistochemical detection of bromodeoxyuridine in formalin-fixed tissues. Histochemistry. 1986; 85(3):193-5. DOI: 10.1007/BF00494803. View

19.

Meyer J . The concept and significance of growth rates in human pulmonary tumors. Ann Thorac Surg. 1972; 14(3):309-22. DOI: 10.1016/s0003-4975(10)65234-4. View

20.

Field D, Fitzgerald P, Sin F . Nucleolar silver-staining patterns related to cell cycle phase and cell generation of PHA-stimulated human lymphocytes. Cytobios. 1984; 41(161):23-33. View