Simultaneous Analysis of Hepatotoxic Pyrrolizidine Alkaloids and N-oxides in Comfrey Root by LC-ion Trap Mass Spectrometry

Overview

Journal Analyst

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2004 Jan 31

PMID 14752559

Citations 8

Authors

Jorgelina C A Wuilloud

Samuel R Gratze

Bryan M Gamble

Karen A Wolnik

Affiliations

Soon will be listed here.

Abstract

The purpose of the current study was to develop a LC-MS(n) method for the analysis of pyrrolizidine alkaloids (PAs) in comfrey. Published data presents an extensive list of PAs and their N-oxides present in comfrey. However, standards are not commercially available for any of the PAs typically present in comfrey. Those PAs that are not stereoisomers were readily resolved on a C(18) column using a water-acetonitrile gradient as the mobile phase. The use of a selective technique, LC-MS/MS, allowed us to identify groups of PAs and their N-oxides, as well as identify the number of PAs present in each group, including those that were not completely resolved chromatographically.

Citing Articles

Pyrrolizidine Alkaloids as Hazardous Toxins in Natural Products: Current Analytical Methods and Latest Legal Regulations.

Lis-Cieplak A, Trzesniowska K, Stolarczyk K, Stolarczyk E Molecules. 2024; 29(14).

PMID: 39064851 PMC: 11279032. DOI: 10.3390/molecules29143269.

In vitro-in silico study on the influence of dose, fraction bioactivated and endpoint used on the relative potency value of pyrrolizidine alkaloid N-oxides compared to parent pyrrolizidine alkaloids.

Alhejji Y, Widjaja F, Tian S, Hoekstra T, Wesseling S, Rietjens I Curr Res Toxicol. 2024; 6:100160.

PMID: 38469320 PMC: 10926302. DOI: 10.1016/j.crtox.2024.100160.

LC-MS/MS Evaluation of Pyrrolizidine Alkaloids Profile in Relation to Safety of Comfrey Roots and Leaves from Polish Sources.

Kimel K, Godlewska S, Glensk M, Gobis K, Osko J, Grembecka M Molecules. 2023; 28(16).

PMID: 37630423 PMC: 10459857. DOI: 10.3390/molecules28166171.

Potential Hepatotoxins Found in Herbal Medicinal Products: A Systematic Review.

Quan N, Xuan T, Teschke R Int J Mol Sci. 2020; 21(14).

PMID: 32708570 PMC: 7404040. DOI: 10.3390/ijms21145011.

Analysis of Pyrrolizidine Alkaloids in Queensland Honey: Using Low Temperature Chromatography to Resolve Stereoisomers and Identify Botanical Sources by UHPLC-MS/MS.

Hungerford N, Carter S, Anuj S, Tan B, Hnatko D, Martin C Toxins (Basel). 2019; 11(12).

PMID: 31835836 PMC: 6950414. DOI: 10.3390/toxins11120726.