A Novel Family of Transporters Mediating the Transport of Glutathione Derivatives in Plants

Overview

Journal Plant Physiol

Specialty Physiology

Date 2004 Jan 20

PMID 14730075

Citations 27

Authors

Ming-Yong Zhang

Andree Bourbouloux

Olivier Cagnac

Chittur V Srikanth

Doris Rentsch

Anand K Bachhawat

Serge Delrot

Affiliations

Soon will be listed here.

Abstract

Uptake and compartmentation of reduced glutathione (GSH), oxidized glutathione (GSSG), and glutathione conjugates are important for many functions including sulfur transport, resistance against biotic and abiotic stresses, and developmental processes. Complementation of a yeast (Saccharomyces cerevisiae) mutant (hgt1) deficient in glutathione transport was used to characterize a glutathione transporter cDNA (OsGT1) from rice (Oryza sativa). The 2.58-kb full-length cDNA (AF393848, gi 27497095), which was obtained by screening of a cDNA library and 5'-rapid amplification of cDNA ends-polymerase chain reaction, contains an open reading frame encoding a 766-amino acid protein. Complementation of the hgt1 yeast mutant strain with the OsGT1 cDNA restored growth on a medium containing GSH as the sole sulfur source. The strain expressing OsGT1 mediated [3H]GSH uptake, and this uptake was significantly competed not only by unlabeled GSSG and GS conjugates but also by some amino acids and peptides, suggesting a wide substrate specificity. OsGT1 may be involved in the retrieval of GSSG, GS conjugates, and nitrogen-containing peptides from the cell wall.

Citing Articles

Foliar Application of Sulfur-Containing Compounds-Pros and Cons.

Bouranis D, Chorianopoulou S Plants (Basel). 2023; 12(22).

PMID: 38005690 PMC: 10674314. DOI: 10.3390/plants12223794.

Identification of a new mutant allele of that regulates kernel development and nutritional quality in maize.

Wang Y, Zhang X, Luo B, Hu H, Zhong H, Zhang H Mol Breed. 2023; 42(2):7.

PMID: 37309320 PMC: 10248714. DOI: 10.1007/s11032-022-01278-9.

Genome-Wide Identification and Transcript Analysis Reveal Potential Roles of Oligopeptide Transporter Genes in Iron Deficiency Induced Cadmium Accumulation in Peanut.

Wang C, Wang X, Li J, Guan J, Tan Z, Zhang Z Front Plant Sci. 2022; 13:894848.

PMID: 35646039 PMC: 9131082. DOI: 10.3389/fpls.2022.894848.

Cysteine and Methionine Biosynthetic Enzymes Have Distinct Effects on Seed Nutritional Quality and on Molecular Phenotypes Associated With Accumulation of a Methionine-Rich Seed Storage Protein in Rice.

Whitcomb S, Rakpenthai A, Bruckner F, Fischer A, Parmar S, Erban A Front Plant Sci. 2020; 11:1118.

PMID: 32793268 PMC: 7387578. DOI: 10.3389/fpls.2020.01118.

Genome-wide analysis indicates diverse physiological roles of the turnip ( var. ) oligopeptide transporters gene family.

Pu Y, Yang D, Yin X, Wang Q, Chen Q, Yang Y Plant Divers. 2018; 40(2):57-67.

PMID: 30159543 PMC: 6091929. DOI: 10.1016/j.pld.2018.03.001.

References

Lu Y, Li Z, Rea P . AtMRP1 gene of Arabidopsis encodes a glutathione S-conjugate pump: isolation and functional definition of a plant ATP-binding cassette transporter gene. Proc Natl Acad Sci U S A. 1997; 94(15):8243-8. PMC: 21588. DOI: 10.1073/pnas.94.15.8243. View

Lu Y, Li Z, Drozdowicz Y, Hortensteiner S, Martinoia E, Rea P . AtMRP2, an Arabidopsis ATP binding cassette transporter able to transport glutathione S-conjugates and chlorophyll catabolites: functional comparisons with Atmrp1. Plant Cell. 1998; 10(2):267-82. PMC: 143980. DOI: 10.1105/tpc.10.2.267. View

Koh S, Wiles A, Sharp J, Naider F, Becker J, Stacey G . An oligopeptide transporter gene family in Arabidopsis. Plant Physiol. 2002; 128(1):21-9. PMC: 148940. View

Liu K, Huang C, Tsay Y . CHL1 is a dual-affinity nitrate transporter of Arabidopsis involved in multiple phases of nitrate uptake. Plant Cell. 1999; 11(5):865-74. PMC: 144217. DOI: 10.1105/tpc.11.5.865. View

KYTE J, Doolittle R . A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982; 157(1):105-32. DOI: 10.1016/0022-2836(82)90515-0. View

Wu J, Maehara T, Shimokawa T, Yamamoto S, Harada C, Takazaki Y . A comprehensive rice transcript map containing 6591 expressed sequence tag sites. Plant Cell. 2002; 14(3):525-35. PMC: 150576. DOI: 10.1105/tpc.010274. View

Isnard A, Thomas D . The study of methionine uptake in Saccharomyces cerevisiae reveals a new family of amino acid permeases. J Mol Biol. 1996; 262(4):473-84. DOI: 10.1006/jmbi.1996.0529. View

Bolchi A, Petrucco S, Tenca P, Foroni C, Ottonello S . Coordinate modulation of maize sulfate permease and ATP sulfurylase mRNAs in response to variations in sulfur nutritional status: stereospecific down-regulation by L-cysteine. Plant Mol Biol. 1999; 39(3):527-37. DOI: 10.1023/a:1006148815106. View

Hauser M, Donhardt A, Barnes D, Naider F, Becker J . Enkephalins are transported by a novel eukaryotic peptide uptake system. J Biol Chem. 2000; 275(5):3037-41. DOI: 10.1074/jbc.275.5.3037. View

10.

Vernoux T, Wilson R, Seeley K, Reichheld J, Muroy S, Brown S . The ROOT MERISTEMLESS1/CADMIUM SENSITIVE2 gene defines a glutathione-dependent pathway involved in initiation and maintenance of cell division during postembryonic root development. Plant Cell. 2000; 12(1):97-110. PMC: 140217. DOI: 10.1105/tpc.12.1.97. View

11.

Bourgis F, Roje S, Nuccio M, Fisher D, Tarczynski M, Li C . S-methylmethionine plays a major role in phloem sulfur transport and is synthesized by a novel type of methyltransferase. Plant Cell. 1999; 11(8):1485-98. PMC: 144290. DOI: 10.1105/tpc.11.8.1485. View

12.

Leustek T, Martin M, Bick J, Davies J . PATHWAYS AND REGULATION OF SULFUR METABOLISM REVEALED THROUGH MOLECULAR AND GENETIC STUDIES. Annu Rev Plant Physiol Plant Mol Biol. 2004; 51:141-165. DOI: 10.1146/annurev.arplant.51.1.141. View

13.

Jamai A, Tommasini R, Martinoia E, Delrot S . Characterization of Glutathione Uptake in Broad Bean Leaf Protoplasts. Plant Physiol. 1996; 111(4):1145-1152. PMC: 160990. DOI: 10.1104/pp.111.4.1145. View

14.

Sherrill C, Fahey R . Import and metabolism of glutathione by Streptococcus mutans. J Bacteriol. 1998; 180(6):1454-9. PMC: 107044. DOI: 10.1128/JB.180.6.1454-1459.1998. View

15.

Iantomasi T, Favilli F, Marraccini P, Magaldi T, Bruni P, Vincenzini M . Glutathione transport system in human small intestine epithelial cells. Biochim Biophys Acta. 1998; 1330(2):274-83. DOI: 10.1016/s0005-2736(97)00097-7. View

16.

Bourbouloux A, Shahi P, Chakladar A, Delrot S, Bachhawat A . Hgt1p, a high affinity glutathione transporter from the yeast Saccharomyces cerevisiae. J Biol Chem. 2000; 275(18):13259-65. DOI: 10.1074/jbc.275.18.13259. View

17.

Foyer C, Theodoulou F, Delrot S . The functions of inter- and intracellular glutathione transport systems in plants. Trends Plant Sci. 2001; 6(10):486-92. DOI: 10.1016/s1360-1385(01)02086-6. View

18.

Fu H, Luan S . AtKuP1: a dual-affinity K+ transporter from Arabidopsis. Plant Cell. 1998; 10(1):63-73. PMC: 143931. DOI: 10.1105/tpc.10.1.63. View

19.

Rennenberg H, Schmitz K, Bergmann L . Long-distance transport of sulfur in Nicotiana tabacum. Planta. 2013; 147(1):57-62. DOI: 10.1007/BF00384591. View

20.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View