Preparation and Property Analysis of a Hepatocyte Targeting PH-sensitive Liposome

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2004 Jan 13

PMID 14716832

Citations 2

Authors

Si-Yuan Wen

Xiao-Hong Wang

Li Lin

Wei Guan

Sheng-Qi Wang

Affiliations

Soon will be listed here.

Abstract

Aim: To develop a hepatocyte targeting pH-sensitive liposome for drug delivery based on active targeting technology mediated by asialoglycoprotein receptors.

Methods: Four types of targeting molecules with galactose residue were synthesized and mixed with pH-sensitive lipids DC-chol/DOPE to prepare liposome with integrated property of hepatocyte specificity and pH sensitivity. Liposome 18-gal was selected with the best transfection activity through cellular uptake experiment. Property analysis was made through experiments of competitive inhibition of receptors, red blood cell hemolysis, in vitro cytotoxicity test by MTS assay and mediation of inhibitory effects of antisense phosphorothioate ODN on gene expression, etc.

Results: Liposome 18-gal had the desired properties of hepatocyte specificity, pH sensitivity, low cytotoxicity, and high transfection efficiency.

Conclusion: Liposome 18-gal can be further developed as a potential hepatocyte- targeting delivery system.

Citing Articles

Immuno-Oncotherapeutic Approaches in Advanced Hepatocellular Carcinoma.

Park R, Eshrat F, Al-Jumayli M, Saeed A, Saeed A Vaccines (Basel). 2020; 8(3).

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Nano-vectors for efficient liver specific gene transfer.

Pathak A, Vyas S, Gupta K Int J Nanomedicine. 2008; 3(1):31-49.

PMID: 18488414 PMC: 2526359.

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