Cyclooxygenase Expression During Helicobacter Pylori Infection in Mongolian Gerbils

Overview

Journal Dig Dis Sci

Specialty Gastroenterology

Date 2004 Jan 7

PMID 14705819

Citations 14

Authors

Taro Sakai

Hirokazu Fukui

Franchesco Franceschi

Rebacca Penland

Antonia R Sepulveda

Takahiro Fujimori

Akira Terano

Robert M Genta

David Y Graham

Yoshio Yamaoka

Affiliations

Soon will be listed here.

Abstract

We investigated the expression of cyclooxygenase-2 (COX-2) and COX-1 mRNAs during longterm Helicobacter pylori infection of the Mongolian gerbil (18 months) as well as the effect of eradication therapy and the cag pathogenicity island on COX mRNA expression. COX mRNA levels were determined by reverse transcription-polymerase chain reaction. Pyloric channel ulcers were noted in one of 10 gerbils (10%) at 3 months, 33% at 6 months, 50% at 9 months, 17% at 10 months, 40% at 12 months and 25% at 18 months after inoculation of parental strains. Nineteen of 21 gerbils had successful H. pylori eradication and showed significant reduction of inflammation and no ulcerations. There were no significant differences in COX-1 mRNA expression between the groups. COX-2 mRNA expression was significantly increased 1 and 3 months after inoculation and then decreased to basal levels. In control animals, COX-2 mRNA expression was significantly higher at 12 and 18 months compared to younger animals. cagE knockout mutants did not induce gastric inflammation and induced significantly lower COX-2 mRNA expression compared to parental strains. COX-2 mRNA was induced early in H. pylori infection and then declined. COX-2 mRNA expression was also induced with aging.

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References

Hla T, Neilson K . Human cyclooxygenase-2 cDNA. Proc Natl Acad Sci U S A. 1992; 89(16):7384-8. PMC: 49714. DOI: 10.1073/pnas.89.16.7384. View

OSullivan M, Huggins Jr E, Meade E, Dewitt D, McCall C . Lipopolysaccharide induces prostaglandin H synthase-2 in alveolar macrophages. Biochem Biophys Res Commun. 1992; 187(2):1123-7. DOI: 10.1016/0006-291x(92)91313-f. View

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

Coyne D, NICKOLS M, Bertrand W, Morrison A . Regulation of mesangial cell cyclooxygenase synthesis by cytokines and glucocorticoids. Am J Physiol. 1992; 263(1 Pt 2):F97-102. DOI: 10.1152/ajprenal.1992.263.1.F97. View

Takahashi S, Fujita T, Yamamoto A . Role of cyclooxygenase-2 in Helicobacter pylori- induced gastritis in Mongolian gerbils. Am J Physiol Gastrointest Liver Physiol. 2000; 279(4):G791-8. DOI: 10.1152/ajpgi.2000.279.4.G791. View

Nakamura M, Takahashi S, Matsui H, Nishikawa K, Akiba Y, Ishii H . Persistent increase in myofibroblasts in Helicobacter heilmannii-infected mice but not in Helicobacter pylori-infected Mongolian gerbils: colocalization of COX-2 and bFGF immunoreactivity. Aliment Pharmacol Ther. 2002; 16 Suppl 2:174-9. DOI: 10.1046/j.1365-2036.16.s2.26.x. View

Robert A, NEZAMIS J, Lancaster C, Hanchar A . Cytoprotection by prostaglandins in rats. Prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic NaCl, and thermal injury. Gastroenterology. 1979; 77(3):433-43. View

Ernst P, Crowe S, Reyes V . How does Helicobacter pylori cause mucosal damage? The inflammatory response. Gastroenterology. 1997; 113(6 Suppl):S35-42; discussion S50. DOI: 10.1016/s0016-5085(97)80009-1. View

Yamaoka Y, Kita M, Kodama T, Sawai N, Imanishi J . Helicobacter pylori cagA gene and expression of cytokine messenger RNA in gastric mucosa. Gastroenterology. 1996; 110(6):1744-52. DOI: 10.1053/gast.1996.v110.pm8964399. View

10.

Yamagata K, Andreasson K, Kaufmann W, Barnes C, Worley P . Expression of a mitogen-inducible cyclooxygenase in brain neurons: regulation by synaptic activity and glucocorticoids. Neuron. 1993; 11(2):371-86. DOI: 10.1016/0896-6273(93)90192-t. View

11.

Fu J, Masferrer J, Seibert K, Raz A, NEEDLEMAN P . The induction and suppression of prostaglandin H2 synthase (cyclooxygenase) in human monocytes. J Biol Chem. 1990; 265(28):16737-40. View

12.

Eberhart C, DuBois R . Eicosanoids and the gastrointestinal tract. Gastroenterology. 1995; 109(1):285-301. DOI: 10.1016/0016-5085(95)90296-1. View

13.

Fellows I, Bhaskar N, Hawkey C . Nature and time-course of piroxicam-induced injury to human gastric mucosa. Aliment Pharmacol Ther. 1989; 3(5):481-8. DOI: 10.1111/j.1365-2036.1989.tb00239.x. View

14.

Yamaoka Y, Kita M, Kodama T, Sawai N, Tanahashi T, Kashima K . Chemokines in the gastric mucosa in Helicobacter pylori infection. Gut. 1998; 42(5):609-17. PMC: 1727111. DOI: 10.1136/gut.42.5.609. View

15.

Ogura K, Maeda S, Nakao M, Watanabe T, Tada M, Kyutoku T . Virulence factors of Helicobacter pylori responsible for gastric diseases in Mongolian gerbil. J Exp Med. 2000; 192(11):1601-10. PMC: 2193104. DOI: 10.1084/jem.192.11.1601. View

16.

Watanabe T, Tada M, Nagai H, Sasaki S, Nakao M . Helicobacter pylori infection induces gastric cancer in mongolian gerbils. Gastroenterology. 1998; 115(3):642-8. DOI: 10.1016/s0016-5085(98)70143-x. View

17.

Hirayama F, Takagi S, Kusuhara H, Iwao E, Yokoyama Y, Ikeda Y . Induction of gastric ulcer and intestinal metaplasia in mongolian gerbils infected with Helicobacter pylori. J Gastroenterol. 1996; 31(5):755-7. DOI: 10.1007/BF02347631. View

18.

McCarthy C, Crofford L, Greenson J, Scheiman J . Cyclooxygenase-2 expression in gastric antral mucosa before and after eradication of Helicobacter pylori infection. Am J Gastroenterol. 1999; 94(5):1218-23. DOI: 10.1111/j.1572-0241.1999.01070.x. View

19.

Schmassmann A, Peskar B, Stettler C, Netzer P, Stroff T, Flogerzi B . Effects of inhibition of prostaglandin endoperoxide synthase-2 in chronic gastro-intestinal ulcer models in rats. Br J Pharmacol. 1998; 123(5):795-804. PMC: 1565228. DOI: 10.1038/sj.bjp.0701672. View

20.

Dixon M, Genta R, Yardley J, Correa P . Classification and grading of gastritis. The updated Sydney System. International Workshop on the Histopathology of Gastritis, Houston 1994. Am J Surg Pathol. 1996; 20(10):1161-81. DOI: 10.1097/00000478-199610000-00001. View