RNAi-mediated Targeting of Heterochromatin by the RITS Complex

Overview

Journal Science

Specialty Science

Date 2004 Jan 6

PMID 14704433

Citations 545

Authors

Andre Verdel

Songtao Jia

Scott Gerber

Tomoyasu Sugiyama

Steven Gygi

Shiv I S Grewal

Danesh Moazed

Affiliations

Soon will be listed here.

Abstract

RNA interference (RNAi) is a widespread silencing mechanism that acts at both the posttranscriptional and transcriptional levels. Here, we describe the purification of an RNAi effector complex termed RITS (RNA-induced initiation of transcriptional gene silencing) that is required for heterochromatin assembly in fission yeast. The RITS complex contains Ago1 (the fission yeast Argonaute homolog), Chp1 (a heterochromatin-associated chromodomain protein), and Tas3 (a novel protein). In addition, the complex contains small RNAs that require the Dicer ribonuclease for their production. These small RNAs are homologous to centromeric repeats and are required for the localization of RITS to heterochromatic domains. The results suggest a mechanism for the role of the RNAi machinery and small RNAs in targeting of heterochromatin complexes and epigenetic gene silencing at specific chromosomal loci.

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