Expression Pattern of the CCAAT/enhancer-binding Proteins C/EBP-alpha, C/EBP-beta and C/EBP-delta in the Human Placenta

Overview

Journal Virchows Arch

Specialties Cell Biology
Molecular Biology
Pathology

Date 2003 Dec 24

PMID 14691721

Citations 8

Authors

Ana-Maria Bamberger

Antonis Makrigiannakis

Martina Schroder

Christoph M Bamberger

Constantine Relakis

Birgit Gellersen

Karin Milde-Langosch

Thomas Loning

Affiliations

Soon will be listed here.

Abstract

The human trophoblast has the capacity to invade maternal tissue in a controlled fashion and to produce a wide range of hormones. The transcription factors belonging to the CCAAT/enhancer-binding protein (C/EBP) family are regulators of intracellular processes and mediators of hormone action. C/EBP binding sites have been described in the promoters of several placenta-expressed target genes. In the present study, we used immunohistochemistry and Western-blot analysis to investigate the expression pattern of the three most important members of this family, C/EBP-alpha, -beta, and -delta, in the normal human placenta as well as in isolated trophoblast cell populations. We found C/EBP-alpha and C/EBP-beta expression in the villous syncytiotrophophoblast (ST) and the extravillous (intermediate) trophoblast (EVT), but it was absent from the villous cytotrophoblast (CT). Interestingly, expression of C/EBP-beta continued to be very strong up to the third trimester of pregnancy, especially in the ST. C/EBP-delta showed overall lower expression levels, stronger only in the EVT, while CT/ST showed very low/negative expression. These data show for the first time the expression pattern and tissue localization of C/EBP factors in the human placenta, indicating that these factors (especially C/EBP-beta) may play important roles in the regulation of placenta-specific genes and processes.

Citing Articles

Noncoding RNA-regulated gain-of-function of STOX2 in Finnish pre-eclamptic families.

Oudejans C, Poutsma A, Michel O, Thulluru H, Mulders J, van de Vrugt H Sci Rep. 2016; 6:32129.

PMID: 27555360 PMC: 4995371. DOI: 10.1038/srep32129.

Landscape of transcriptional deregulations in the preeclamptic placenta.

Vaiman D, Calicchio R, Miralles F PLoS One. 2013; 8(6):e65498.

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CCAAT-enhancer-binding protein β (C/EBPβ) and downstream human placental growth hormone genes are targets for dysregulation in pregnancies complicated by maternal obesity.

Vakili H, Jin Y, Menticoglou S, Cattini P J Biol Chem. 2013; 288(31):22849-61.

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CCAAT/enhancer-binding proteins are key regulators of human type two deiodinase expression in a placenta cell line.

Canettieri G, Santaguida M, Antonucci L, Della Guardia M, Franchi A, Coni S Endocrinology. 2012; 153(8):4030-8.

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Ectopic expression of hC/EBPs in breast tumor cells induces apoptosis.

Pal P, Lochab S, Kanaujiya J, Sanyal S, Trivedi A Mol Cell Biochem. 2009; 337(1-2):111-8.

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