Shigella Dysenteriae Type 1-specific Bacteriophage from Environmental Waters in Bangladesh

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2003 Dec 9

PMID 14660345

Citations 8

Authors

Shah M Faruque

Nityananda Chowdhury

Rasel Khan

M Rubayet Hasan

Jebun Nahar

M Johirul Islam

Shinji Yamasaki

A N Ghosh

G Balakrish Nair

David A Sack

Affiliations

Soon will be listed here.

Abstract

Shigella dysenteriae type 1 is the causative agent of the most severe form of bacillary dysentery, which occurs as epidemics in many developing countries. We isolated a bacteriophage from surface water samples from Bangladesh that specifically lyses strains of S. dysenteriae type 1. This phage, designated SF-9, belongs to the Podoviridae family and has a 41-kb double-stranded DNA genome. Further screening of water samples for the prevalence of the phage revealed 9 of 71 (12.6%) water samples which were positive for the phage. These water samples were also positive in PCR assays for one or more S. dysenteriae type 1-specific genes, including ipaBCD and stx1, and live S. dysenteriae type 1 was isolated from three phage-positive samples. The results of this study suggest that phage SF-9 may have epidemiological applications in tracing the presence of S. dysenteriae type 1 in environmental waters.

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