Antioxidant Ability and Lipid Peroxidation in the Hippocampus with Epileptogenesis Induced by Fe3+ Injection into the Amygdaloid Body of Rats

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 2003 Dec 3

PMID 14649733

Citations 4

Authors

Yuto Ueda

Akira Nakajima

Jun Tokumaru

Affiliations

Soon will be listed here.

Abstract

To analyze antioxidant ability and lipid peroxidation in the hippocampus of rats in an interictal state of FeCl3-induced epileptogenesis, the hippocampal eliminating decay ratio of exogenously applied nitroxide radical (3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (carbamoyl-PROXYL)) by electron paramagnetic resonance (EPR) spectroscopy, and the thiobarbituric reactive substances (TBARS) level in the hippocampus were measured. The prolonged half-life of electron paramagnetism of carbamoyl-PROXYL in the hippocampus of rats with chronic FeCl3-induced epileptogenesis revealed decreased antioxidant ability, which supports the vulnerability against oxidative stress. In addition, TBARS level (marker of lipid peroxidation) was increased in the hippocampus of rats injected with FeCl3 compared with that of control. This study revealed that repetitive seizures resulted in the decreased hippocampal antioxidant ability with lipid peroxidation and explained the regional vulnerability to oxidative stress in the limbic system with epileptogenesis.

Citing Articles

Identification of miRNA-mRNA regulatory network associated with the glutamatergic system in post-traumatic epilepsy rats.

Zhang X, Ma Y, Zhou F, Zhang M, Zhao D, Wang X Front Neurol. 2023; 13:1102672.

PMID: 36619916 PMC: 9822725. DOI: 10.3389/fneur.2022.1102672.

Identification of MicroRNA-Potassium Channel Messenger RNA Interactions in the Brain of Rats With Post-traumatic Epilepsy.

Li Z, Ma Y, Zhou F, Jia X, Zhan J, Tan H Front Mol Neurosci. 2021; 13:610090.

PMID: 33597846 PMC: 7882489. DOI: 10.3389/fnmol.2020.610090.

Hippocampal gene expression profiling in a rat model of posttraumatic epilepsy reveals temporal upregulation of lipid metabolism-related genes.

Ueda Y, Kitamoto A, Willmore L, Kojima T Neurochem Res. 2013; 38(7):1399-406.

PMID: 23585123 DOI: 10.1007/s11064-013-1037-9.

Functional role for redox in the epileptogenesis: molecular regulation of glutamate in the hippocampus of FeCl3-induced limbic epilepsy model.

Ueda Y, Doi T, Nagatomo K, Willmore L, Nakajima A Exp Brain Res. 2007; 181(4):571-7.

PMID: 17486325 DOI: 10.1007/s00221-007-0954-8.

References

Hiramatsu M, Liu J, Edamatsu R, Ohba S, Kadowaki D, Mori A . Probucol scavenged 1,1-diphenyl-2-picrylhydrazyl radicals and inhibited formation of thiobarbituric acid reactive substances. Free Radic Biol Med. 1994; 16(2):201-6. DOI: 10.1016/0891-5849(94)90144-9. View

Willmore L, Rubin J . Formation of malonaldehyde and focal brain edema induced by subpial injection of FeCl2 into rat isocortex. Brain Res. 1982; 246(1):113-9. DOI: 10.1016/0006-8993(82)90147-0. View

Willmore L . Post-traumatic epilepsy: cellular mechanisms and implications for treatment. Epilepsia. 1990; 31 Suppl 3:S67-73. DOI: 10.1111/j.1528-1157.1990.tb05861.x. View

Willmore L, Triggs W . Iron-induced lipid peroxidation and brain injury responses. Int J Dev Neurosci. 1991; 9(2):175-80. DOI: 10.1016/0736-5748(91)90009-b. View

Tokumaru J, Ueda Y, Yokoyama H, Nakajima A, Doi T, Mitsuyama Y . In vivo evaluation of hippocampal anti-oxidant ability of zonisamide in rats. Neurochem Res. 2000; 25(8):1107-11. DOI: 10.1023/a:1007622129369. View

Triggs W, Willmore L . In vivo lipid peroxidation in rat brain following intracortical Fe2+ injection. J Neurochem. 1984; 42(4):976-80. DOI: 10.1111/j.1471-4159.1984.tb12699.x. View

MCCAY P . Vitamin E: interactions with free radicals and ascorbate. Annu Rev Nutr. 1985; 5:323-40. DOI: 10.1146/annurev.nu.05.070185.001543. View

Gluck M, Jayatilleke E, Shaw S, Rowan A, Haroutunian V . CNS oxidative stress associated with the kainic acid rodent model of experimental epilepsy. Epilepsy Res. 2000; 39(1):63-71. DOI: 10.1016/s0920-1211(99)00111-4. View

Hamada H, Hiramatsu M, Edamatsu R, Mori A . Free radical scavenging action of baicalein. Arch Biochem Biophys. 1993; 306(1):261-6. DOI: 10.1006/abbi.1993.1509. View

10.

Doi T, Ueda Y, Tokumaru J, Mitsuyama Y, Willmore L . Sequential changes in glutamate transporter mRNA levels during Fe(3+)-induced epileptogenesis. Brain Res Mol Brain Res. 2000; 75(1):105-12. DOI: 10.1016/s0169-328x(99)00303-4. View

11.

Willmore L, Hiramatsu M, Kochi H, Mori A . Formation of superoxide radicals after FeCl3 injection into rat isocortex. Brain Res. 1983; 277(2):393-6. DOI: 10.1016/0006-8993(83)90954-x. View

12.

Volterra A, Trotti D, Floridi S, Racagni G . Reactive oxygen species inhibit high-affinity glutamate uptake: molecular mechanism and neuropathological implications. Ann N Y Acad Sci. 1994; 738:153-62. DOI: 10.1111/j.1749-6632.1994.tb21800.x. View

13.

Liu J, Edamatsu R, Kabuto H, Mori A . Antioxidant action of guilingji in the brain of rats with FeCl3-induced epilepsy. Free Radic Biol Med. 1990; 9(5):451-4. DOI: 10.1016/0891-5849(90)90023-c. View

14.

Volterra A, Trotti D, Racagni G . Glutamate uptake is inhibited by arachidonic acid and oxygen radicals via two distinct and additive mechanisms. Mol Pharmacol. 1994; 46(5):986-92. View

15.

Racine R . Modification of seizure activity by electrical stimulation. II. Motor seizure. Electroencephalogr Clin Neurophysiol. 1972; 32(3):281-94. DOI: 10.1016/0013-4694(72)90177-0. View

16.

Zhang Y, Fung L . The roles of ascorbic acid and other antioxidants in the erythrocyte in reducing membrane nitroxide radicals. Free Radic Biol Med. 1994; 16(2):215-22. DOI: 10.1016/0891-5849(94)90146-5. View

17.

Ueda Y, Doi T, Tokumaru J, Yokoyama H, Nakajima A, Mitsuyama Y . Collapse of extracellular glutamate regulation during epileptogenesis: down-regulation and functional failure of glutamate transporter function in rats with chronic seizures induced by kainic acid. J Neurochem. 2001; 76(3):892-900. DOI: 10.1046/j.1471-4159.2001.00087.x. View

18.

Ueda Y, Willmore L . Sequential changes in glutamate transporter protein levels during Fe(3+)-induced epileptogenesis. Epilepsy Res. 2000; 39(3):201-9. DOI: 10.1016/s0920-1211(99)00122-9. View

19.

Lipton S, Choi Y, Pan Z, Lei S, Chen H, Sucher N . A redox-based mechanism for the neuroprotective and neurodestructive effects of nitric oxide and related nitroso-compounds. Nature. 1993; 364(6438):626-32. DOI: 10.1038/364626a0. View

20.

Fuchs J, Groth N, Herrling T, Zimmer G . Electron paramagnetic resonance studies on nitroxide radical 2,2,5,5-tetramethyl-4-piperidin-1-oxyl (TEMPO) redox reactions in human skin. Free Radic Biol Med. 1997; 22(6):967-76. DOI: 10.1016/s0891-5849(96)00433-9. View