Distinct Downstream Signaling Mechanism Between Erythropoietin Receptor and Interleukin-2 Receptor

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1992 Dec 1

PMID 1464316

Citations 9

Authors

Y Yamamura

Y Kageyama

T Matuzaki

M Noda

Y Ikawa

Affiliations

Soon will be listed here.

Abstract

Erythropoietin receptor (EPOR) and interleukin-2 receptor beta chain (IL-2R beta) belong to the same cytokine receptor superfamily and have highly conserved sequences in their intracellular signaling domain. However, common downstream signaling pathways of these receptors have not been demonstrated. In the present study, we introduced and expressed the murine EPOR in murine IL-2-, IL-3- and IL-5-dependent cell lines and analyzed their growth response to EPO. We found that the expression of EPOR induced EPO dependence in IL-3-dependent BAF-B03 and IL-5-dependent Y16 cells but not in IL-2-dependent CTLL-2 cells, although the EPOR-expressing CTLL-2 cell lines could bind and internalize EPO as efficiently as the BAF-B03-derived cell lines. Additional expression of AIC2B, a common signal transducer for IL-3R, IL-5R and GM-CSFR, made no difference to the EPO responsiveness of the EPOR-expressing CTLL-2 cell lines. These results suggest that the cellular components required for the transduction of EPOR signal and IL-2R signal are at least partially different, and this difference cannot be explained solely by the absence of AIC2B.

Citing Articles

Augmentation of NK Cell Proliferation and Anti-tumor Immunity by Transgenic Expression of Receptors for EPO or TPO.

Chanswangphuwana C, Allan D, Chakraborty M, Reger R, Childs R Mol Ther. 2020; 29(1):47-59.

PMID: 33010232 PMC: 7791015. DOI: 10.1016/j.ymthe.2020.09.023.

Epo receptors are not detectable in primary human tumor tissue samples.

Elliott S, Swift S, Busse L, Scully S, Van G, Rossi J PLoS One. 2013; 8(7):e68083.

PMID: 23861852 PMC: 3701640. DOI: 10.1371/journal.pone.0068083.

The effect of erythropoietin on normal and neoplastic cells.

Elliott S, Sinclair A Biologics. 2012; 6:163-89.

PMID: 22848149 PMC: 3402043. DOI: 10.2147/BTT.S32281.

Absence of an essential role for thymic stromal lymphopoietin receptor in murine B-cell development.

Carpino N, Thierfelder W, Chang M, Saris C, Turner S, Ziegler S Mol Cell Biol. 2004; 24(6):2584-92.

PMID: 14993294 PMC: 355866. DOI: 10.1128/MCB.24.6.2584-2592.2004.

Absence of cytokine receptor-dependent specificity in red blood cell differentiation in vivo.

Goldsmith M, Mikami A, You Y, Liu K, Thomas L, Pharr P Proc Natl Acad Sci U S A. 1998; 95(12):7006-11.

PMID: 9618529 PMC: 22719. DOI: 10.1073/pnas.95.12.7006.

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