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Characterization of a Protective Monoclonal Antibody Recognizing Staphylococcus Aureus MSCRAMM Protein Clumping Factor A

Overview
Journal Infect Immun
Publisher American Society for Microbiology
Specialty Allergy & Immunology
Date 2003 Nov 26
PMID 14638774
Citations 63
Authors
Andrea E Hall
Paul J Domanski
Pratiksha R Patel
John H Vernachio
Peter J Syribeys
Elena L Gorovits
Michael A Johnson
Julia M Ross
Jeff T Hutchins
Joseph M Patti
Affiliations
Soon will be listed here.
Abstract

The Staphylococcus aureus MSCRAMM (microbial surface components recognizing adhesive matrix molecules) protein clumping factor A (ClfA) has been shown to be a critical virulence factor in several experimental models of infection. This report describes the generation, characterization, and in vivo evaluation of a murine monoclonal antibody (MAb) against ClfA. Flow cytometric analysis revealed that MAb 12-9 recognized ClfA protein expressed by all of the clinical S. aureus strains obtained from a variety of sources. In assays measuring whole-cell S. aureus binding to human fibrinogen, MAb 12-9 inhibited S. aureus binding by over 90% and displaced up to 35% of the previously adherent S. aureus bacteria. Furthermore, a single infusion of MAb 12-9 was protective against an intravenous challenge with a methicillin-resistant strain of S. aureus in a murine sepsis model (P < 0.0001). These data suggest that anti-ClfA MAb 12-9 should be further investigated as a novel immunotherapy for the treatment and prevention of life-threatening S. aureus infections.

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