Expression of CC and CXC Chemokines and Chemokine Receptors in Human Leprosy Skin Lesions

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 2003 Nov 25

PMID 14632750

Citations 13

Authors

A A Kirkaldy

A C Musonda

S Khanolkhar-Young

S Suneetha

D N J Lockwood

Affiliations

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Abstract

We have investigated the expression of chemokines and their receptors in leprosy skin lesions using immunohistochemistry. Skin biopsies from 25 leprosy patients across the leprosy spectrum, 11 patients undergoing type I reversal reactions and four normal donors were immunostained by ABC peroxidase method using antibodies against CC and CXC chemokines and their receptors. Using an in situ hybridization technique we have also studied the expression of monocyte chemoattractant protein 1 (MCP-1), RANTES and interleukin (IL)-8 chemokines mRNA in leprosy skin lesions. Chemokines and receptor expression was detected in all leprosy skin biopsies. Expression of CC chemokines MCP-1 (P < 0.01) and RANTES (P < 0.01) were elevated significantly in borderline tuberculoid leprosy in reversal reaction compared to non-reactional borderline tuberculoid leprosy, but there was no difference in the expression of IL-8 chemokine. Surprisingly, there was no significant difference in the expression of CC (CCR2 and CCR5) and CXC (CXCR2) chemokine receptors across the leprosy spectrum. Similarly, there was no significant difference in the expression of mRNA for MCP-1, regulated upon activation normal T cell expressed and secreted (RANTES) and IL-8 chemokines. Here, the presence of a neutrophil chemoattractant IL-8 in leprosy lesions, which do not contain neutrophils, suggests strongly a role of IL-8 as a monocyte and lymphocyte recruiter in leprosy lesions. These results suggest that the chemokines and their receptors, which are known to chemoattract T lymphocytes and macrophages, are involved in assembling the cellular infiltrate found in lesions across the leprosy spectrum.

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References

RIDLEY D, Jopling W . Classification of leprosy according to immunity. A five-group system. Int J Lepr Other Mycobact Dis. 1966; 34(3):255-73. View

Fantuzzi L, Canini I, Belardelli F, Gessani S . IFN-beta stimulates the production of beta-chemokines in human peripheral blood monocytes. Importance of macrophage differentiation. Eur Cytokine Netw. 2002; 12(4):597-603. View

Volc-Platzer B, Stemberger H, Luger T, Radaszkiewicz T, WIEDERMANN G . Defective intralesional interferon-gamma activity in patients with lepromatous leprosy. Clin Exp Immunol. 1988; 71(2):235-40. PMC: 1541447. View

Mehra V, Modlin R . T-lymphocytes in leprosy lesions. Curr Top Microbiol Immunol. 1990; 155:97-109. DOI: 10.1007/978-3-642-74983-4_7. View

Rollins B, Walz A, Baggiolini M . Recombinant human MCP-1/JE induces chemotaxis, calcium flux, and the respiratory burst in human monocytes. Blood. 1991; 78(4):1112-6. View

Yamamura M, Uyemura K, Deans R, Weinberg K, Rea T, Bloom B . Defining protective responses to pathogens: cytokine profiles in leprosy lesions. Science. 1991; 254(5029):277-9. DOI: 10.1126/science.254.5029.277. View

Rathanaswami P, Hachicha M, Sadick M, Schall T, McColl S . Expression of the cytokine RANTES in human rheumatoid synovial fibroblasts. Differential regulation of RANTES and interleukin-8 genes by inflammatory cytokines. J Biol Chem. 1993; 268(8):5834-9. View

Schulz B, Michel G, Wagner S, Suss R, Beetz A, Peter R . Increased expression of epidermal IL-8 receptor in psoriasis. Down-regulation by FK-506 in vitro. J Immunol. 1993; 151(8):4399-406. View

Baggiolini M, DEWALD B, Moser B . Interleukin-8 and related chemotactic cytokines--CXC and CC chemokines. Adv Immunol. 1994; 55:97-179. View

10.

Woodroofe M, Cuzner M . Cytokine mRNA expression in inflammatory multiple sclerosis lesions: detection by non-radioactive in situ hybridization. Cytokine. 1993; 5(6):583-8. DOI: 10.1016/s1043-4666(05)80008-0. View

11.

Wechsler A, Gordon M, Dendorfer U, LeClair K . Induction of IL-8 expression in T cells uses the CD28 costimulatory pathway. J Immunol. 1994; 153(6):2515-23. View

12.

Kasahara K, Tobe T, Tomita M, Mukaida N, Matsushima K, Yoshida T . Selective expression of monocyte chemotactic and activating factor/monocyte chemoattractant protein 1 in human blood monocytes by Mycobacterium tuberculosis. J Infect Dis. 1994; 170(5):1238-47. DOI: 10.1093/infdis/170.5.1238. View

13.

Lemster B, Carroll P, Rilo H, Johnson N, Nikaein A, Thomson A . IL-8/IL-8 receptor expression in psoriasis and the response to systemic tacrolimus (FK506) therapy. Clin Exp Immunol. 1995; 99(2):148-54. PMC: 1534299. DOI: 10.1111/j.1365-2249.1995.tb05525.x. View

14.

Bacon K, Premack B, Gardner P, Schall T . Activation of dual T cell signaling pathways by the chemokine RANTES. Science. 1995; 269(5231):1727-30. DOI: 10.1126/science.7569902. View

15.

Schroder J, Noso N, Sticherling M, Christophers E . Role of eosinophil-chemotactic C-C chemokines in cutaneous inflammation. J Leukoc Biol. 1996; 59(1):1-5. View

16.

Kunkel S, Lukacs N, Kasama T, Strieter R . The role of chemokines in inflammatory joint disease. J Leukoc Biol. 1996; 59(1):6-12. DOI: 10.1002/jlb.59.1.6. View

17.

Standiford T, Kunkel S, Greenberger M, Laichalk L, Strieter R . Expression and regulation of chemokines in bacterial pneumonia. J Leukoc Biol. 1996; 59(1):24-8. DOI: 10.1002/jlb.59.1.24. View

18.

Li J, Ireland G, Farthing P, Thornhill M . Epidermal and oral keratinocytes are induced to produce RANTES and IL-8 by cytokine stimulation. J Invest Dermatol. 1996; 106(4):661-6. DOI: 10.1111/1523-1747.ep12345482. View

19.

Ritter U, Moll H, Laskay T, Brocker E, Velazco O, Becker I . Differential expression of chemokines in patients with localized and diffuse cutaneous American leishmaniasis. J Infect Dis. 1996; 173(3):699-709. DOI: 10.1093/infdis/173.3.699. View

20.

Taub D, Key M, Longo D, Murphy W . Chemokines and T lymphocyte activation: I. Beta chemokines costimulate human T lymphocyte activation in vitro. J Immunol. 1996; 156(6):2095-2103. View