Spatiotemporal Changes of Coxsackievirus and Adenovirus Receptor in Rat Hearts During Postnatal Development and in Cultured Cardiomyocytes of Neonatal Rat

Overview

Journal Virchows Arch

Specialties Cell Biology
Molecular Biology
Pathology

Date 2003 Nov 19

PMID 14624362

Citations 14

Authors

Takeshi Kashimura

Makoto Kodama

Yuko Hotta

Junichi Hosoya

Kaori Yoshida

Takuya Ozawa

Ritsuo Watanabe

Yuji Okura

Kiminori Kato

Haruo Hanawa

Ryozo Kuwano

Yoshifusa Aizawa

Affiliations

Soon will be listed here.

Abstract

Coxsackievirus B is the most common cause of viral myocarditis and is particularly virulent in neonates and children. Adenovirus is also a leading cause of the disease. The determinant of tropism for both viruses is considered to be the expression of coxsackievirus and adenovirus receptor (CAR) in target organs. However, developmental change and physiological localization of CAR in the heart are unknown. We examined expression levels of CAR in rat hearts by quantitative real-time polymerase chain reaction and Western blot analysis and found that CAR decreased gradually during postnatal development, although CAR was detectable, even in adults. Immunohistochemistry revealed CAR on the whole surface of cardiomyocytes in immature rat hearts. In contrast, CAR was detected predominantly on intercalated disks in the adult heart and was accumulated especially at the contact point between the cultured cardiomyocytes, even though they were prepared from the neonatal rat heart. In conclusion, CAR was expressed abundantly on the whole surface of cardiomyocytes in immature rat hearts. Both the expression level and the localization of CAR are possible determinants of the susceptibility to viral myocarditis of neonates and children.

Citing Articles

Coxsackievirus and Adenovirus Receptor (CAR) Expression in Autopsy Tissues: Organ-Specific Patterns and Clinical Significance.

Ramamoorthy S, Garg S, Mishra B, Radotra B, Nahar Saikia U Cureus. 2023; 15(4):e37138.

PMID: 37153286 PMC: 10159945. DOI: 10.7759/cureus.37138.

CAR links hypoxia signaling to improved survival after myocardial infarction.

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The IgCAM CAR Regulates Gap Junction-Mediated Coupling on Embryonic Cardiomyocytes and Affects Their Beating Frequency.

Matthaeus C, Juttner R, Gotthardt M, Rathjen F Life (Basel). 2023; 13(1).

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CXADR: From an Essential Structural Component to a Vital Signaling Mediator in Spermatogenesis.

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Vascular Endothelial Integrity Affects the Severity of Enterovirus-Mediated Cardiomyopathy.

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