Involvement of a Pertussis Toxin-sensitive G-protein in Excitation-contraction Coupling of Intact and Cut-end Voltage-clamped Skeletal Muscle Fibres

Overview

Journal Pflugers Arch

Specialty Physiology

Date 1992 Aug 1

PMID 1461719

Authors

A Mouzou

J P Poindessault

G Raymond

Affiliations

Soon will be listed here.

Abstract

In voltage-clamped frog muscle fibres 10 ng/ml PTX induced a decrease (approximately 35%) of tension when applied externally. Internal application in cut-end fibres significantly depressed tension after 20 min. This effect increased with time to reach 65% after 60 min. PTX shifted the voltage-dependent inactivation curve of tension by 30 mV towards hyperpolarizations and this was counteracted by raising external calcium concentration. The toxin induced a parallel decrease in tension and voltage-sensitive charge movement (49 +/- 9% and 52 +/- 6% respectively; n = 6). This was not counteracted by prior impregnation with forskolin. Internally applied GTP gamma S (500 microM) induced a simultaneous increase in tension (57 +/- 5%) and charge amount displaced (40 +/- 7%). By contrast, GDP beta S decreased tension and charge movement by 35 +/- 5% and 36 +/- 6% respectively.

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