The VTI Family of SNARE Proteins is Necessary for Plant Viability and Mediates Different Protein Transport Pathways

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 2003 Nov 15

PMID 14615598

Citations 95

Authors

Marci Surpin

Haiyan Zheng

Miyo T Morita

Cheiko Saito

Emily Avila

Joshua J Blakeslee

Anindita Bandyopadhyay

Valentina Kovaleva

David Carter

Angus Murphy

Masao Tasaka

Natasha Raikhel

Affiliations

Soon will be listed here.

Abstract

The Arabidopsis genome contains a family of v-SNAREs: VTI11, VTI12, and VTI13. Only VTI11 and VTI12 are expressed at appreciable levels. Although these two proteins are 60% identical, they complement different transport pathways when expressed in the yeast vti1 mutant. VTI11 was identified recently as the mutated gene in the shoot gravitropic mutant zig. Here, we show that the vti11 zig mutant has defects in vascular patterning and auxin transport. An Arabidopsis T-DNA insertion mutant, vti12, had a normal phenotype under nutrient-rich growth conditions. However, under nutrient-poor conditions, vti12 showed an accelerated senescence phenotype, suggesting that VTI12 may play a role in the plant autophagy pathway. VTI11 and VTI12 also were able to substitute for each other in their respective SNARE complexes, and a double-mutant cross between zig and vti12 was embryo lethal. These results suggest that some VTI1 protein was necessary for plant viability and that the two proteins were partially functionally redundant.

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