The Laccase Gene Family in Coprinopsis Cinerea (Coprinus Cinereus)

Overview

Journal Curr Genet

Specialty Genetics

Date 2003 Nov 6

PMID 14600788

Citations 15

Authors

Patrik J Hoegger

Monica Navarro-Gonzalez

Sreedhar Kilaru

Matthias Hoffmann

Elisha D Westbrook

Ursula Kues

Affiliations

Soon will be listed here.

Abstract

In this study, we isolated and sequenced eight non-allelic laccase genes from Coprinopsis cinerea ( Coprinus cinereus) homokaryon AmutBmut. These eight genes represent the largest laccase gene family identified so far in a single haploid fungal genome. We analyzed the phylogenetic relationships between these genes by intron positions, amino acid sequence conservation and similarities in promoter sequences. All deduced protein products have the laccase signature sequences L1-L4, the typical conserved cysteine and the ten histidine residues which are ligands in the two laccase copper-binding centers, T1 and T2/T3. Proteins Lcc2 and Lcc3 of Coprinopsis cinerea are most similar to the acidic, membrane-associated laccase CLAC2 from Coprinellus congregatus implicated in neutralization of acidic medium. All other laccases from the saprophyte Coprinopsis cinerea, including the well described enzyme Lcc1, form a cluster separate from these three enzymes and from various laccases of wood-rotting and plant-pathogenic basidiomycetes.

Citing Articles

Comparative genomic analysis of five coprinoid mushrooms species.

Wang J, Zhang R, Ding G, Wang L, Wang W, Zhang Y Funct Integr Genomics. 2023; 23(2):159.

PMID: 37178396 PMC: 10182949. DOI: 10.1007/s10142-023-01094-0.

Improvement of laccase activity by silencing PacC in Ganoderma lucidum.

Zhu J, Song S, Lian L, Shi L, Ren A, Zhao M World J Microbiol Biotechnol. 2022; 38(2):32.

PMID: 34989903 DOI: 10.1007/s11274-021-03216-x.

Alternative Splicing of Heat Shock Transcription Factor 2 Regulates the Expression of Laccase Gene Family in Response to Copper in .

Zhang Y, Wu Y, Yang X, Yang E, Xu H, Chen Y Appl Environ Microbiol. 2021; 87(8).

PMID: 33579682 PMC: 8091107. DOI: 10.1128/AEM.00055-21.

Signal peptide replacement resulted in recombinant homologous expression of laccase Lcc8 in Coprinopsis cinerea.

Schulze M, Geisler L, Majcherczyk A, Ruhl M AMB Express. 2019; 9(1):36.

PMID: 30874916 PMC: 6420550. DOI: 10.1186/s13568-019-0761-1.

Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation.

Yang J, Li W, Ng T, Deng X, Lin J, Ye X Front Microbiol. 2017; 8:832.

PMID: 28559880 PMC: 5432550. DOI: 10.3389/fmicb.2017.00832.

References

Leonowicz A, Trojanowski J . Induction of laccase by ferulic acid in basidiomycetes. Acta Biochim Pol. 1975; 22(4):291-5. View

Yaver D, Golightly E . Cloning and characterization of three laccase genes from the white-rot basidiomycete Trametes villosa: genomic organization of the laccase gene family. Gene. 1996; 181(1-2):95-102. DOI: 10.1016/s0378-1119(96)00480-5. View

Otterbein L, Record E, Longhi S, Asther M, Moukha S . Molecular cloning of the cDNA encoding laccase from Pycnoporus cinnabarinus I-937 and expression in Pichia pastoris. Eur J Biochem. 2000; 267(6):1619-25. DOI: 10.1046/j.1432-1327.2000.01166.x. View

Piontek K, Antorini M, Choinowski T . Crystal structure of a laccase from the fungus Trametes versicolor at 1.90-A resolution containing a full complement of coppers. J Biol Chem. 2002; 277(40):37663-9. DOI: 10.1074/jbc.M204571200. View

Fernandez-Larrea J, Stahl U . Isolation and characterization of a laccase gene from Podospora anserina. Mol Gen Genet. 1996; 252(5):539-51. DOI: 10.1007/BF02172400. View

Eggert C, Lafayette P, Temp U, Eriksson K, Dean J . Molecular analysis of a laccase gene from the white rot fungus Pycnoporus cinnabarinus. Appl Environ Microbiol. 1998; 64(5):1766-72. PMC: 106228. DOI: 10.1128/AEM.64.5.1766-1772.1998. View

Nosanchuk J, Casadevall A . The contribution of melanin to microbial pathogenesis. Cell Microbiol. 2003; 5(4):203-23. DOI: 10.1046/j.1462-5814.2003.00268.x. View

Kues U . Life history and developmental processes in the basidiomycete Coprinus cinereus. Microbiol Mol Biol Rev. 2000; 64(2):316-53. PMC: 98996. DOI: 10.1128/MMBR.64.2.316-353.2000. View

Kumar S, Phale P, Durani S, Wangikar P . Combined sequence and structure analysis of the fungal laccase family. Biotechnol Bioeng. 2003; 83(4):386-94. DOI: 10.1002/bit.10681. View

10.

Weaver R, Rajagopalan K, Handler P, Jeffs P, Byrne W, Rosenthal D . Isolation of -L-glutaminyl 4-hydroxybenzene and -L-glutaminyl 3,4-benzoquinone: a natural sulfhydryl reagent, from sporulating gill tissue of the mushroom Agaricus bisporus. Proc Natl Acad Sci U S A. 1970; 67(2):1050-6. PMC: 283311. DOI: 10.1073/pnas.67.2.1050. View

11.

Bottoli A, Boulianne R, Granado J, Aebi M, Kues U . Rapid isolation of genes from an indexed genomic library of C. cinereus in a novel pab1+ cosmid. J Microbiol Methods. 1999; 35(2):129-41. DOI: 10.1016/s0167-7012(98)00109-2. View

12.

Marbach K, Fernandez-Larrea J, Stahl U . Reversion of a long-living, undifferentiated mutant of Podospora anserina by copper. Curr Genet. 1994; 26(2):184-6. DOI: 10.1007/BF00313809. View

13.

Giardina P, Cannio R, Martirani L, Marzullo L, Palmieri G, Sannia G . Cloning and sequencing of a laccase gene from the lignin-degrading basidiomycete Pleurotus ostreatus. Appl Environ Microbiol. 1995; 61(6):2408-13. PMC: 167512. DOI: 10.1128/aem.61.6.2408-2413.1995. View

14.

Heinzkill M, Bech L, Halkier T, Schneider P, Anke T . Characterization of laccases and peroxidases from wood-rotting fungi (family Coprinaceae). Appl Environ Microbiol. 1998; 64(5):1601-6. PMC: 106202. DOI: 10.1128/AEM.64.5.1601-1606.1998. View

15.

Hakulinen N, Kiiskinen L, Kruus K, Saloheimo M, Paananen A, Koivula A . Crystal structure of a laccase from Melanocarpus albomyces with an intact trinuclear copper site. Nat Struct Biol. 2002; 9(8):601-5. DOI: 10.1038/nsb823. View

16.

Hermann T, KURTZ M, Champe S . Laccase localized in hulle cells and cleistothecial primordia of Aspergillus nidulans. J Bacteriol. 1983; 154(2):955-64. PMC: 217550. DOI: 10.1128/jb.154.2.955-964.1983. View

17.

Giardina P, Aurilia V, Cannio R, Marzullo L, Amoresano A, Siciliano R . The gene, protein and glycan structures of laccase from Pleurotus ostreatus. Eur J Biochem. 1996; 235(3):508-15. DOI: 10.1111/j.1432-1033.1996.00508.x. View

18.

Iakovlev A, Stenlid J . Spatiotemporal Patterns of Laccase Activity in Interacting Mycelia of Wood-Decaying Basidiomycete Fungi. Microb Ecol. 2002; 39(3):236-245. DOI: 10.1007/s002480000022. View

19.

Soden D, Dobson A . Differential regulation of laccase gene expression in Pleurotus sajor-caju. Microbiology (Reading). 2001; 147(Pt 7):1755-1763. DOI: 10.1099/00221287-147-7-1755. View

20.

Marchuk D, Drumm M, Saulino A, Collins F . Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Res. 1991; 19(5):1154. PMC: 333800. DOI: 10.1093/nar/19.5.1154. View