Quantitative Metabolome Analysis Using Capillary Electrophoresis Mass Spectrometry

Overview

Journal J Proteome Res

Publisher American Chemical Society

Specialty Biochemistry

Date 2003 Oct 30

PMID 14582645

Citations 295

Authors

Tomoyoshi Soga

Yoshiaki Ohashi

Yuki Ueno

Hisako Naraoka

Masaru Tomita

Takaaki Nishioka

Affiliations

Soon will be listed here.

Abstract

A new approach for the comprehensive and quantitative analysis of charged metabolites by capillary electrophoresis mass spectrometry (CE-MS) is proposed. Metabolites are first separated by CE based on charge and size and then selectively detected using MS by monitoring over a large range of m/z values. This method enabled the determination of 352 metabolic standards and its utility was demonstrated in the analysis of 1692 metabolites from Bacillus subtilis extracts, revealing significant changes in metabolites during B. subtilis sporulation.

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