Dual Targeting and Function of a Protease in Mitochondria and Chloroplasts

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2003 Oct 28

PMID 14578924

Citations 16

Authors

Shashi Bhushan

Benoit Lefebvre

Annelie Stahl

Sarah J Wright

Barry D Bruce

Marc Boutry

Elzbieta Glaser

Affiliations

Soon will be listed here.

Abstract

Here we show, using the green fluorescent protein (GFP) fusion system, that an Arabidopsis thaliana zinc-metalloprotease (AtZn-MP) is targeted to both mitochondria and chloroplasts. A deletion mutant lacking the amino-terminal 28 residues, with translation initiation at the second methionine residue, was imported into chloroplasts only. However, a mutated form of the full-length targeting peptide, in which the second methionine residue is changed to leucine, was imported to both organelles. No GFP fluorescence was detected when a frame-shift mutation was introduced between the first and second ATG codons of the Zn-MP-GFP construct, suggesting no alternative translational initiation. Our results show that the dual targeting of the Zn-MP is due to an ambiguous targeting peptide. Furthermore, we show that the recombinant AtZn-MP degrades mitochondrial and chloroplastic targeting peptides, indicating its function as a signal peptide degrading protease in both mitochondria and chloroplasts.

Citing Articles

Chloroplast Proteases: Updates on Proteolysis within and across Suborganellar Compartments.

Nishimura K, Kato Y, Sakamoto W Plant Physiol. 2016; 171(4):2280-93.

PMID: 27288365 PMC: 4972267. DOI: 10.1104/pp.16.00330.

PtrA/NINV, an alkaline/neutral invertase gene of Poncirus trifoliata, confers enhanced tolerance to multiple abiotic stresses by modulating ROS levels and maintaining photosynthetic efficiency.

Dahro B, Wang F, Peng T, Liu J BMC Plant Biol. 2016; 16:76.

PMID: 27025596 PMC: 4812658. DOI: 10.1186/s12870-016-0761-0.

Interaction of the dual targeting peptide of Thr-tRNA synthetase with the chloroplastic receptor Toc34 in Arabidopsis thaliana.

Ye W, Spanning E, Glaser E, Maler L FEBS Open Bio. 2015; 5:405-12.

PMID: 26101739 PMC: 4430637. DOI: 10.1016/j.fob.2015.04.014.

Organellar oligopeptidase (OOP) provides a complementary pathway for targeting peptide degradation in mitochondria and chloroplasts.

Kmiec B, Teixeira P, Berntsson R, Murcha M, Branca R, Radomiljac J Proc Natl Acad Sci U S A. 2013; 110(40):E3761-9.

PMID: 24043784 PMC: 3791733. DOI: 10.1073/pnas.1307637110.

Mitochondrial biogenesis and function in Arabidopsis.

Millar A, Small I, Day D, Whelan J Arabidopsis Book. 2012; 6:e0111.

PMID: 22303236 PMC: 3243404. DOI: 10.1199/tab.0111.

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