Functional Characterization of Genes Involved in Alkane Oxidation by Pseudomonas Aeruginosa

Overview

Journal Antonie Van Leeuwenhoek

Publisher Springer

Specialty Microbiology

Date 2003 Oct 24

PMID 14574114

Citations 25

Authors

Theo H M Smits

Bernard Witholt

Jan B van Beilen

Affiliations

Soon will be listed here.

Abstract

Most clinical isolates identified as Pseudomonas aeruginosa grow on long-chain n-alkanes, while environmental P. aeruginosa isolates often grow on medium- as well as long-chain n-alkanes. Heterologous expression showed that the two alkane hydroxylase homologs of P. aeruginosa PAO1 (AlkB1 and AlkB2) oxidize C(12)-C(16)n-alkanes, while two rubredoxin (RubA1 and RubA2) and a rubredoxin reductase (RubB) homologs can replace their P. putida GPo1 counterparts in n-octane oxidation. The two long-chain alkane hydroxylase genes are present in all environmental and clinical isolates of P. aeruginosa strains tested in this study.

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